Summary
Growth of influenza virus type A2, strain Japan/305/57 (formerly grown in the allantoic cavity of 11-day old chick embryos), was obtained in cultures of chick embryo fibroblasts. Virus, passed four times in chick embryo fibroblasts, followed by one passage in the allantoic sac of 11-day old embryonated eggs, gave clear plaques in a chick embryo monolayer under a serum-free agar overlay with an added DEAE-dextran concentration of 0.5 mg./ml. After three days incubation at 37° C in stoppered flasks the plaques reached a diameter of 2–3 mm. An approximately straight-line dose-response relationship was observed between the square root of the number of plaques produced and the relative concentration of virus. Specificity was demonstrated by quantitative neutralization with immune serum.
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Peters, A., Goedemans, W.T. A sensitive plaque assay for influenza virus A2 Japan in chick embryo fibroblast cultures. Archiv f Virusforschung 19, 190–196 (1966). https://doi.org/10.1007/BF01241498
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DOI: https://doi.org/10.1007/BF01241498