Summary
Analyses of the polypeptide composition of influenza B viruses by 13 per cent SDS-polyacrylamide gel electrophoresis are reported.
The viruses contained polypeptides of eight species ranging in molecular weight from 27,000 to 78,000. Four of them were glycopeptides and were selectively removed from the surface of the virion by Bromelain treatment. One of the glycopeptides was identified as viral neuraminidase.
Three antigenically distinct strains of influenza virus, B/Lee/40, B/Massachusetts/1/71 and B/Hong Kong/5/72, showed an essentially identical electrophoretic picture, although strain-to-strain difference was observed in the migration rate of HA1 and HA2 polypeptides.
Similar content being viewed by others
References
Chakraverty, P.: Antigenic relationship between influenza B viruses. Bull. Wld. Hlth. Org.45, 755–766 (1971).
Chakraverty, P.: Antigenic relationships between the neuraminidase of influenza B virus. Bull. Wld. Hlth. Org.46, 473–476 (1972).
Kilbourne, E. D., Choppin, P. W., Schulze, I. T., Scholtissek, C., Bucher, D. L.: Influenza virus polypeptides and antigens—summary of influenza workshop I. J. infect. Dis.125, 447–455 (1972).
Lazarowitz, S. G., Compans, R. W., Choppin, P. W.: Proteolytic cleavage of the hemagglutinin polypeptide of influenza virus. Function of the uncleaved polypeptide HA. Virology52, 109–212 (1973).
Laver, W. G., Baker, N.: Amino acid composition of polypeptides from influenza virus particles. J. gen. Virol.17, 61–67 (1972).
Lazdins, I., Haslam, E. A., White, D. O.: The polypeptides of influenza virus. VI. Composition of the neuraminidase. Virology49, 758–765 (1972).
Maizel, J. V.: Polyacrylamide gel electrophoresis of viral protein. In: Methods in Virology (Maramorosch, K., Koprowski, H., eds.), Vol. 5, pp. 179–246. New York-London: Academic Press, 1971.
Oxford, J. S.: Polypeptide composition of influenza B viruses and enzymes associated with the purified virus particles. J. Virol.12, 827–835 (1973).
Riley, H. D., Jr.: Reye's syndrome. J. infect. Dis.125, 77–81 (1972).
Schild, G. C., Pereira, M. S., Chakraverty, P., Coleman, M. T., Dowdle, W. R., Chang, W. K.: Antigenic variation of influenza B virus. Brit. med. J.4, 127 to 131 (1973).
Tobita, K., Kilbourne, E. D.: Genetic recombination for antigenic markers of antigenically different strains of influenza B virus. J. Virol.13, 347–352 (1974).
Wrigley, N. G., Skehel, J. J., Charlwood, P. A., Brand, C. M.: The size and shape of influenza virus neuraminidase. Virology51, 525–529 (1973).
Author information
Authors and Affiliations
Additional information
With 5 Figures
Rights and permissions
About this article
Cite this article
Tobita, K., Kilbourne, E.D. Structural polypeptides of antigenically distinct strains of influenza B virus. Archives of Virology 47, 367–374 (1975). https://doi.org/10.1007/BF01347978
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01347978