Zusammenfassung
U-Zellen (permanente, menschliche Amnionzellen) lassen sich durch Human-interferon (HIF) aus peripheren Leukocyten gegenüber Vermehrung von Semliki Forest Virus (SFV) gut schützen. Im Gegensatz zur genusspezifischen Wirkung von Interferon läßt sich in Mäuse-L929-Zellkulturen (permanente Mäusefibroblastenlinie) ebenfalls ein Schutzeffekt beobachten. Die antivirale Aktivität von HIF gegenüber sechs weiteren Primatenzellkulturen wurde untersucht. Besonders auffallend ist dabei, daß die beiden lymphoblastoiden Zellinien RPMI1788 und Kaplan nicht geschützt werden können.
Der Vergleich der Sensitivität von 10 verschiedenen Viren gegenüber HIF in primären Nierenzellkulturen grüner Meerkatzen ergibt eine Abstufungsreihe mit SFV als sensitivstem und zwei Vacciniavirusstämmen als kaum hemmbaren Vertretern. Hierbei überrascht die geringe Hemmbarkeit der Vacciniavirusstämme im Vergleich zu Herpes simplex Virus Typ 1.
Summary
U cells (a permanent, human amnion cell line) were protected against infection with Semliki Forest Virus (SFV) by human interferon (HIF) from peripheral leukocytes. Despite the usual genus-specific action of interferons, mouse L929 cultures (a permanent mouse fibroblast cell line) were also protected by HIF. The antiviral action of HIF in six other primate cell cultures was also examined. It is of interest that two lymphoblastoid cell lines, RPMI 1788 and Kaplan, were insensitive to HIF.
The sensitivity of ten different viruses against HIF in primary African green monkey kidney cell cultures was compared. Among the viruses tested SFV was the most sensitive whereas two strains of vaccinia virus were barely inhibited. In contrast, type 1 herpes simplex virus was relatively sensitive to the action of HIF.
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Literatur
Berman, B., Vilćek, J.: Cellular binding characteristics of human interferon. Virology57, 378–386 (1974).
De Clercq, E., Stewart II, W. E.: The breadth of interferon action. In: Selective inhibitors of viral functions (W. A. Carter, ed.), 81–106. Cleveland: CRC Press 1973.
Desmyter, J., Rawls, W. E., Melnick, J. L.: A human interferon that crosses the species line. Proc. Nat. Acad. Sci.59, 69–76 (1968).
Diehl, V., Henle, G., Henle, W., Kohn, G.: Demonstration of herpes group virus in cultures of peripheral leukocytes from patients with infectious mononucleosis. J. Virol.2, 663–669 (1968).
Duc-Goiran, P., Galliot, B., Chany, C.: Studies on virus-induced interferons produced by the human amniotic membrane and white blood cells. Arch. ges. Virusforsch.34, 232–243 (1971).
Floyd, R., Glasser, R., Vonka, V., Benyesh-Melnick, M.: Studies on the growth of herpes simplex virus in lymphoblastoid cells. Acta virol.15, 133–142 (1971).
Glasgow, L. A., Hanshaw, J. B., Merigan, T. C., Petralli, J. K.: Interferon and cytomegalovirusin vivo andin vitro. Proc. Soc. exp. Biol. (N.Y.)125, 843–849 (1967).
Gresser, I., Bandu, M.-T., Brouty-Boye, D., Tovey, M.: Pronounced antiviral activity of human interferon on bovine and porcine cells. Nature (Lond.)251, 543–545 (1974).
Henle, W., Henle, G., zur Hausen, H.: Effect of herpes simples virus on cultured Burkitt tumor cells and its failure to influence the Epstein-Barr virus carrier state. Cancer Res.29, 489–494 (1969).
Hilfenhaus, J., Karges, H. E.: Growth inhibition of human lymphoblastoid cells by interferon preparations, obtained from human leukocytes. Z. Naturforsch.29 c, 618–622 (1974).
Hilfenhaus, J., Karges, H. E., Weinmann, E., Barth, R.: Effect of administered human interferon on experimental rabies in monkeys. Infect. Immun.11, 1156 (1975).
Ho, M., Enders, J. F.: Further studies on an inhibitor of viral activity appearing in infected cell cultures and its role in chronic viral infections. Virology9, 446–477 (1959).
Laufs, R., Steinke, H., Jacobs, C., Hilfenhaus, J., Karges, H.: Influence of interferon on the replication of oncogenic herpesviruses in tissue cultures and in nonhuman primates. Med. Microbiol. Immunol.160, 285–294 (1974).
Levy-Koenig, R. E., Golgher, R. R., Paucker, K.: Immunology of interferons. II. Heterospecific activities of human interferons and their neutralization by antibody. J. Immunol.104, 791–797 (1970).
Lockart, R. Z., Jr.: Criteria for acceptance of a viral inhibitor as an interferon and a general description of the biological properties of known interferons. In: Interferon and interferon inducers (N. B. Finter, ed.), 11–27. Amsterdam: North-Holland Publishing Company 1973.
Majer, M., Plotkin, S. A.: Strains of human viruses. Basel: S. Karger 1972.
Morton, H. J.: A survey of commercially available tissue culture media.In vitro 6, 89–108 (1970).
Neumann-Haefelin, D., Sundmacher, R., Sauter, B.: Antiviral treatment with human leukocyte interferon: effect on herpes simplex and vaccinia keratitis in monkeys. Med. Microbiol. Immunol. Im Druck.
Siewers, C. M. F., John, C. E., Medearis, D. N., Jr.: Sensitivity of human cell strains to interferon. Proc. Soc. exp. Biol. (N.Y.)133, 1178–1183 (1970).
Stewart II, W. E., Lockart, R. Z., Jr.: Relative antiviral resistance induced in homologous and heterologous cells by cross-reacting interferons. J. Virol.6, 795 to 799 (1970).
Stewart II, W. E., de Clercq, E., de Somer, P.: Recovery of cell-bound interferon. J. Virol.10, 707–712 (1972).
Youngner, J. S., Thacore, H. R., Kelly, M. E.: Sensitivity of ribonucleic acid and deoxyribonucleic acid viruses to different species of interferon in cell cultures. J. Virol.10, 171–178 (1972).
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Hilfenhaus, J., Thierfelder, H. & Barth, R. Sensitivität verschiedener Primatenzellen und animaler Viren gegenüber der antiviralen Aktivität von menschlichem Leukocyteninterferon. Archives of Virology 48, 203–211 (1975). https://doi.org/10.1007/BF01317963
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DOI: https://doi.org/10.1007/BF01317963