Summary
A plaque assay method using ASFV previously adapted to growth in chick embryo fibroblasts is described. Chick embryo fibroblast monolayers under bactoagar or methylcellulose have been employed using cysteine, arginine, DEAE-Dextran and HEPES as additives. Plaque production was optimal under methylcellulose. HEPES rendered the plaques more clear when used with the overlay. Arginine enhances plaque formation with bactoagar, and DEAE-Dextran doubles the plaque size. The growth curve of ASFV in chick embryo monolayers has been studied.
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Gil-Fernandez, C., García-Gancedo, A. & Vilas-Minondo, P. Plaque formation by African swine fever virus in chick embryo fibroblasts in the absence of CO2 atmosphere. Archives of Virology 52, 207–216 (1976). https://doi.org/10.1007/BF01348018
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DOI: https://doi.org/10.1007/BF01348018