Summary
Selection of populations of Tacaribe virus strain 11573 lethal for mice was carried out by serial intracerebral passage of the virus in adult mice. Viral populations have been characterized by determination of virulence for suckling, weanling, and adult mice, and by histopathologic changes observed in brains of adult mice after intracerebral inoculation. Some of the virus preparations produced 80 to 90 per cent mortality after two or three intracerebral passages in adult mice and maintained this virulence for 1 to 3 passages, after which the virulence rapidly declined with subsequent passages. Clinical signs of infection in adult mice were manifested by a rough hair-coat, ventriflexed posture, diminished activity, increased excitability, flaccid hind-limb extension with progressive paralysis and death. Histologic examination revealed meningoencephalitis.
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Borden, E. C., Nathanson, N.: Tacaribe virus infection of the mouse: an immunopathologic disease model. Lab. Invest.30, 465–473 (1974).
Buchmeier, M. J., Rawls, W. E.: Variation between strains of hamsters in the lethality of Pichinde virus infections. Infect. Immun.16, 413–421 (1977).
Buckley, S. M., Casals, J.: Lassa fever, a new virus disease of man from West Africa. III. Isolation and characterization of the virus. Amer. J. trop. Med. Hyg.19, 680–691 (1970).
Calisher, C. H., Tzianabos, T., Lord, R. D., Coleman, P. H.: Tamiami virus, a new member of the Tacaribe group. Amer. J. trop. Med. Hyg.19, 520–526 (1970).
Cole, G. A., Gilden, D. H., Monjan, A. A., Nathanson, N.: Lymphocytic choriomeningitis virus: pathogenesis of acute central nervous system disease. Fed. Proc.30, 1831–1841 (1971).
Collins, D. N., Weigand, H., Hotchin, J.: The effects of pretreatment with x-rays on the pathogenesis of lymphocytic choriomeningitis in mice. II. The pathological histology. J. Immunol.87, 682–687 (1961).
Downs, W. G., Anderson, C. R., Spence, L., Aitken, T. H. G., Greenhall, A. H.: Tacaribe virus, a new agent isolated fromArtibeus bats and mosquitoes in Trinidad, West Indies. Amer. J. trop. Med. Hyg.12, 640–646 (1963).
Eddy, G. A., Wagner, F. S., Scott, S. K., Brand, O. M.: Pathogenesis of Machupo virus infection in primates. Bull. WHO52, 517–521 (1975).
Gilden, D. H., Cole, G. A., Nathanson: Immunopathogenesis of acute central nervous system disease produced by lymphocytic choriomeningitis virus. II. Adoptive immunization of virus carriers. J. exp. Med.135, 874–889 (1972).
Johnson, K. M., Wiebenga, N. H., Mackenzie, R. B., Kuns, M. L., Tauraso, N. M., Shelokov, A., Webb, P. A., Justines, G., Beye, H. K.: Virus isolations from human cases of hemorrhagic fever in Bolivia. Proc. Soc. exp. Biol. Med.118, 113–118 (1965).
Lillie, R. D., Armstrong, C.: Pathology of lymphocytic choriomeningitis in mice. Arch. Pathol.40, 141–152 (1945).
Parodi, A. S., Greenway, D. J., Rugiero, H. R., Frigerio, M., de la Barrera, J. M., Mettler, N., Garzon, F., Boxaca, M., Guerrero, L., Nota, N.: Sobre la etiologia del brote epidemico de Junin. Dia Med.30, 2300–2301 (1958).
Pinheiro, F. P., Shope, R. E., Paes de Andrade, A. H., Bensabath, G., Cacios, G. V., Casals, J.: Amapari, a new virus of the Tacaribe group from rodents and mites of Amapa Territory, Brazil. Proc. Soc. exp. Biol. Med.122, 531–535 (1966).
Reed, L. J., Muench, H.: A simple method for estimating fifty per cent end-points. Amer. J. Hyg.27, 493–497 (1938).
Steel, R. G. D., Torrie, J. H.: Enumeration data. II. Contingency tables. In: Principles and Procedures of Statistics, 366–386. New York: McGraw-Hill Book Co. 1960.
Tauraso, N., Shelokov, A.: Protection against Junin virus by immunization with live Tacaribe virus. Proc. Soc. exp. Biol. Med.119, 608–611 (1965).
Trapido, H., Sanmartin, C.: Pichidine virus, a new virus of the Tacaribe group from Colombia. Amer. J. trop. Med. Hyg.20, 631–641 (1965).
Walker, D. H., Wulff, H., Lange, J. V., Murphy, F. A.: Comparative Pathology of Lassa virus infection in monkeys, guinea-pigs, andMastomys natalensis. Bull. WHO52, 523–534 (1975).
Webb, P. A., Johnson, K. M., Hibbs, J. B., Kuns, M. L.: Parana, a new Tacaribe complex virus from Paraguay. Arch. ges. Virusforsch.32, 379–388 (1970).
Webb, P. A., Johnson, K. M., Peters, C. J., Justines, G.: Behavior of Machupo and Latino viruses in Calomys callosus from two geographic areas of Bolivia. In:Lehmann-Grube, F. (ed.), Lymphocytic Choriomeningitis Virus and Other Arneaviruses, 312–322. Berlin-Heidelberg-New York: Springer 1973.
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In conducting the research described in this report, the investigators adhered to the “Guide for the Care and Use of Laboratory Animals”, as promulgated by the Committee on the Revision of the Guide for Laboratory Animal Facilities and Care of the Institute of Laboratory Animal Resources, National Research Council. The facilities are fully accredited by the American Association for Accreditation of Laboratory Animal Care.
The views of the authors do not purport to reflect the positions of the Department of the Army or the Department of Defense.
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Rosato, R.R., Elwell, M.R. & Eddy, G.A. Virulence alterations of Tacaribe virus infection in adult mice: Lethal model for encephalitis. Archives of Virology 58, 137–147 (1978). https://doi.org/10.1007/BF01315406
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DOI: https://doi.org/10.1007/BF01315406