Summary
A sensitive mumps virus plaque neutralization test has been developed based on the potentiation of virus-antibody complexes by heterologous anti-immunoglobulins (AIG). The enhanced neutralization test was approximately 100 times more sensitive than the conventional neutralization test or the hemagglutination-inhibition test. Using AIG against human immunoglobulin G (IgG) or human IgM permitted determination of the relative titers of the two classes of mumps antibody. The test does not require special equipment or expertise and can be readily introduced in virological laboratories.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Albrecht, P., Ennis, F. A., Saltzman, E. J., Krugman, S.: Persistence of maternal antibody in infants beyond 12 months: Mechanism of measles vaccine failure. J. Pediatr.91, 715–718 (1977).
Almeida, J. D., Cinander, B., Howatson, A.: The structure of antigen-antibody complexes. A study by electron microscopy. J. exp. Med.118, 327–349 (1963).
Ashe, W. K., Notkins, A. L.: Neutralization of an infectious herpes simplex virus-antibody complex by anti-γ-globulin. Proc. Nat. Acad. Sci.56, 447–451 (1966).
Black, F. L.: Measles and Mumps. In:Rose, N. R., Friedman, H. (eds.), Manual of Clinical Immunology, 444–447. American Society for Microbiology 1976.
Brown, G., Baublis, J. V., O'Lear, T. P.: Development and duration of mumps fluorescent antibodies in various immunoglobulin fractions of human sera. J. Immunol.104, 86–94 (1970).
Buynak, E. G., Whitman, J. R., Jr., Roem, R. R., Morton, D. H., Lampson, G. P., Hilleman, M. R.: Comparison of neutralization and hemagglutination-inhibiting techniques for measuring mumps antibody. Proc. Soc. exp. Biol. Med.125, 1068–1071 (1967).
Dales, S., Kajioka, R.: The cycle of multiplication of vaccinia virus in Earle's strain L cells. I. Uptake and penetration. Virology24, 278–294 (1964).
Daugharty, H., Warfield, D. T., Hemingway, W. D., Casey, H. L.: Mumps classic-specific immunoglobulins in radio-immunoassay and conventional serology. Infect. Immun.7, 380–385 (1973).
Deinhardt, F., Shramek, G.: Development of an attenuated mumps virus vaccine. I. Determination of neutralizing serum antibodies against mumps virus. J. Immunol.93, 462–465 (1964).
Ennis, F. A.: Immunity to mumps in an institutional epidemic. Correlation of insusceptibility to mumps with serum plaque neutralizing and hemagglutination-inhibiting antibodies. J. inf. Dis.119, 654–657 (1969).
Ennis, F. A., Douglas, R. D., Stewart, G. L., Hopps, H. E., Meyer, H. M., Jr.: A plaque neutralization test for determining mumps antibodies. Proc. Soc. exp. Biol. Med.129, 896–899 (1968).
Forghani, B., Schmidt, N. T., Lennette, E. H.: Sensitivity of a radioimmunoassay method for detection of certain viral antibodies in sera and cerebrospinal fluid. J. clin. Microbiol.4, 470–478 (1976).
Hernandez, R., Just, M., Burgin-Wolff, A.: Screening for mumps immunity with the microtiter solid-phase radioimmunoassay. Infection4, 121–124 (1976).
Hahon, N.: Neutralization of residual infectivity of Venezuelan equine encephalomyelitis virus by anti-gamma globulin. J. gen. Virol.6, 361–372 (1970).
Joklik, W. K.: The intracellular fate of rabbitpox virus rendered non-infectious by various reagents. Virology22, 629–622 (1964).
Kärber, G.: Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche. Arch. exp. Pathol. Pharmacol.162, 480–487 (1931).
Kenny, M. T., Albright, K. L., Sanderson, R. P.: Microneutralization test for the determination of mumps antibody in Vero cells. Appl. Microbiol.20, 371–373 (1970).
Kenny, M. T., Schell, K.: Microassay of measles and mumps virus and antibody in Vero cells. J. biol. Stand.3, 291–306 (1975).
Lehman-Grube, L., Ambrassat, J.: A new method to detect lymphocytic choriomeningitis virus-specific antibody in human sera. J. gen. Virol.37, 85–93 (1977).
Mandel, B.: The interaction of neutralized poliovirus with HeLa cells. II. Elution, penetration, uncoating. Virology31, 248–259 (1967).
Notkins, A. L., Mage, M., Ashe, W. E., Mahar, S.: Neutralization of sensitized lactic dehydrogenase virus by anti-γ-globulin. J. Immunol.100, 314–320 (1968).
Notkins, A. L.: Infectious virus-antibody complexes: Interaction with antiimmunoglobulins, complement, and rheumatoid factor. J. exp. Med.134, 41s-51s (1971).
Porter, D. D., Larsen, A. E.: Aleutian disease of mink: infectious virus antibody complexes in the serum. Proc. Soc. exp. Biol. Med.126, 680–684 (1967).
Radwan, A. I., Burger, D., Davis, W. C.: The fate of sensitized equine arteritis virus following neutralization by complement and anti-IgG serum. Virology53, 372–378 (1973).
Svehag, S. E.: Formation and dissociation of virus-antibody complexes with special reference to the neutralization process. Progr. med. Virol.10, 1–62 (1967).
Wenner, H. A., Monley, A., Jenson, M. H.: A study of infections caused by mumps and Newcastle disease viruses. I. Some properties of specific and nonspecific serum hemagglutination inhibitor components. J. Immunol.68, 357–368 (1952).
Zalan, E., Dorman, E., Labsoffsky, N. E.: Infectious influenza virus-antibody complexes. Arch. ges. Virusforsch.34, 209–213 (1971).
Author information
Authors and Affiliations
Additional information
With 1 Figure
Rights and permissions
About this article
Cite this article
Sato, H., Albrecht, P., Hicks, J.T. et al. Sensitive neutralization test for virus antibody. Archives of Virology 58, 301–311 (1978). https://doi.org/10.1007/BF01317822
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01317822