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Interferon induction with Newcastle disease virus in FS-4 cells: Effect of 5,6-dichloro-1-β-D-ribofuranosylbenzimidazole (DRB)

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Summary

DRB is an inhibitor of heterogeneous nuclear RNA (hnRNA) and messenger RNA (mRNA) synthesis. The effect of DRB on interferon production stimulated by Newcastle disease virus (NDV) in the human FS-4 cells was studied. Interferon production in cells primed by treatment with interferon was markedly enhanced (superinduced) in the presence of DRB. This superinduction was essentially due to an inhibition of the rapid decline (shutoff) of interferon production observed in primed cells not treated with DRB. Continuous presence of DRB was required for maximal superinduction. In this and other respects the interferon response induced by NDV in primed cells resembled poly(I) · poly(C)-induced interferon production. In contrast interferon production in cells not primed with interferon was virtually abolished by DRB treatment. Since neither virus specific RNA synthesis nor virus replication were significantly affected by DRB, the inhibition of interferon production is likely to result from the inhibitory action of DRB on a cellular, rather than viral, function. Apparently some differences exist in the synthesis or processing of the mRNAs for interferons in primed and unprimed cells and these determine the different sensitivities of these two responses to DRB.

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References

  1. Atherton, K. T., Burke, D. C.: Interferon induction by viruses and polynucleotides: A differential effect of camptothecin. J. gen. Virol.29, 297–304 (1975).

    Google Scholar 

  2. Fraser, N. W., Sehgal, P. B., Darnell, J. E.: DRB-induced premature termination of late adenovirus transcription. Nature272, 590–593 (1978).

    Google Scholar 

  3. Kohase, M., Vilček, J.: Regulation of human interferon production stimulated with poly(I) · poly(C): Correlation between shutoff and hyporesponsiveness to reinduction. Virology76, 47–54 (1977).

    Google Scholar 

  4. Kowal, K. J., Youngner, J. S.: Induction of interferon by temperature-sensitive mutants of Newcastle disease virus. Virology90, 90–102 (1978).

    Google Scholar 

  5. Mozes, L. W., Vilček, J.: Distinguishing characteristics of interferon induction with poly(I) · poly(C) and Newcastle disease virus in human cells. Virology65, 100–111 (1975).

    Google Scholar 

  6. Sehgal, P. B., Darnell, J. E., jr., Tamm, I.: The inhibition by DRB (5,6-dichloro-1-β-D-ribofuranosylbenzimidazole) of hnRNA and mRNA production in HeLa cells. Cell9, 473–480 (1976).

    Google Scholar 

  7. Sehgal, P. B., Tamm, I.: An evaluation of messenger RNA competition in the shutoff of human interferon production. Proc. Nat. Acad. Sci. U.S.A.73, 1612 to 1625 (1976).

    Google Scholar 

  8. Sehgal, P. B., Tamm, I.: Halogenated benzimidazole ribosides. Biochem. Pharmacol.27, 2475–2485 (1978).

    Google Scholar 

  9. Sehgal, P. B., Tamm, I.: Two mechanisms contribute to the superinduction of poly(I) · poly(C)-induced human fibroblast interferon production. Virology92, 240–244 (1979).

    Google Scholar 

  10. Sehgal, P. B., Tamm, I., Vilček, J.: Human interferon production: Superinduction by 5,6-dichloro-1-β-D-ribofuranosylbenzimidazole. Science190, 282–284 (1975).

    Google Scholar 

  11. Stewart, W. E., Gosser, L. B., Lockart, R. Z., jr.: Priming: a nonantiviral function of interferon. J. Virol.7, 792–801 (1971).

    Google Scholar 

  12. Tamm, I., Hand, R., Galiguiri, L. A.: Action of dichlorobenzimidazole riboside on RNA synthesis in L-929 and HeLa cells. J. Cell Biol.69, 229–240 (1976).

    Google Scholar 

  13. Tamm, I., Nemes, M. M., Osterhout, S.: On the role of ribonucleic acid in animal virus synthesis. I. Studies with 5,6-dichloro-1-β-D-ribofuranosylbenzimidazole. J. exp. Med.111, 339–349 (1960).

    Google Scholar 

  14. Tan, Y. H., Armstrong, J. A., Ke, Y. H., Ho, M.: Regulation of cellular interferon production: Enhancement by antimetabolites. Proc. Nat. Acad. Sci. U.S.A.67, 464–471 (1970).

    Google Scholar 

  15. Vilček, J.: Metabolic determinants of the induction of interferon by a synthetic double-stranded polynucleotide in rabbit kidney cells. Ann. N.Y. Acad. Sci.173, 390–403 (1970).

    Google Scholar 

  16. Vilček, J., Havell, E. A., Kohase, M.: Superinduction of interferon with metabolic inhibitors: Possible mechanisms and practical applications. J. inf. Dis. (Suppl.)133, A 22-A 29 (1976).

    Google Scholar 

  17. Vilček, J., Rossman, T. G., Varacalli, F.: Differential effects of actinomycin D and puromycin on the release of interferon induced by double stranded RNA. Nature222, 682–683 (1969).

    Google Scholar 

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  1. M. Kohase

    Present address: Department of Measles Virus, National Institute of Health, Murayama Annex, Gauken, Musashimurayama, 190-12, Tokyo, Japan

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  1. Department of Microbiology, New York University, School of Medicine, New York, USA

    M. Kohase & J. Vilček

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  1. M. Kohase
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  2. J. Vilček
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Kohase, M., Vilček, J. Interferon induction with Newcastle disease virus in FS-4 cells: Effect of 5,6-dichloro-1-β-D-ribofuranosylbenzimidazole (DRB). Archives of Virology 62, 263–271 (1979). https://doi.org/10.1007/BF01317558

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  • DOI: https://doi.org/10.1007/BF01317558

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