Skip to main content
SpringerLink
Log in

Role of epidermal Langerhans cells in resistance to herpes simplex virus infection

  • Original Papers
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

To investigate the role of epidermal Langerhans cells (LC) in resistance to herpes simplex virus (HSV) infection, nonadherent spleen cells taken from BALB/c mice immunized with HSV were cultured with syngeneic epidermal cells (EC) and ultraviolet light-inactivated HSV antigen. After five days of culture, T cell-dependent proliferative response was determined by3H-thymidine incorporation. Treatment of EC with anti-Iad monoclonal antibody plus complement before cultivation prevented this proliferation, which suggested that LC induced stimulation of immune T cells. When these stimulated spleen cells were transferred to intracutaneously infected nude mice, the virus titer in the skin was reduced markedly and the formation of zosteriform skin lesions was completely inhibited. On the other hand, transfer of unstimulated cells, which were cultured with HSV antigen only or with HSV antigen and EC treated with anti-Iad monoclonal antibody plus complement, resulted in delayed viral clearance and development of the lesions. These results indicate that LC are of importance as accessory cells in the control of cutaneous HSV infection. Furthermore, Lyt 1+ T cells in the stimulated population were shown to have greater protective activity than Lyt 2+ T cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ahmann, G. B., Sachs, D. H., Hodes, R. J.: Requirement for an I a-bearing accessory cell in Con A-induced T cell proliferation. J. Immunol.121, 1981–1989 (1978).

    Google Scholar 

  2. Black, A. K., Greaves, M. W., Hensby, C. N., Plummer, N. A.: Increased prostaglandin E2 and F2a in human skin at 6 and 24 H after ultraviolet B irradiation. Br. Clin. Pharmacol.5, 431–436 (1978).

    Google Scholar 

  3. Blyth, W. A., Harbour, D. A., Hill, T. J.: Pathogenesis of zosteriform spread of herpes simplex virus in the mouse. J. gen. Virol.65, 1477–1486 (1984).

    Google Scholar 

  4. Blyth, W. A., Hill, T. J., Field, H. J., Harbour, D. H.: Reactivation of herpes simplex virus infection by ultraviolet light and possible involvement of prostaglandins. J. gen. Virol.33, 547–550 (1976).

    Google Scholar 

  5. Braathen, L. R., Bjerche, S., Thorsly, E.: The antigen-presenting function of human Langerhans cells. Immunobiology168, 301–312 (1984).

    Google Scholar 

  6. Elemets, C. A., Bergstresser, P. R., Tigelaar, R. E., Wood, P. J., Streilein, J. W.: Analysis of the mechanism of unresponsiveness produced by haptens painted on skin exposed to low dose ultraviolet radiation. J. Exp. Med.158, 781–794 (1983).

    Google Scholar 

  7. Ennis, J. A., Wells, M.: Immune control of herpes simplex virus infection. Cancer Res.34, 1140–1145 (1974).

    Google Scholar 

  8. Habu, S., Raff, M. C.: Accessory cell dependence of lectin induced proliferation of mouse T lymphocytes. Eur. J. Immunol.7, 451–457 (1977).

    Google Scholar 

  9. Harbour, D. A., Hill, T. J., Blyth, W. A.: Recurrent herpes simplex in the mouse: Inflammation in the skin and activation in the ganglia following peripheral stimulation. J. gen. Virol.64, 1491–1498 (1983).

    Google Scholar 

  10. Iwasaka, T., Sheridan, J. F., Aurelian, L.: Immunity to herpes simplex virus type 2: Recurrent lesions are associated with the induction of suppressor cells and soluble suppressor factors. Infect. Immun.42, 955–964 (1983).

    Google Scholar 

  11. Julis, M. H., Simpson, E., Herzenberg, L. A.: A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur. J. Immunol.3, 645–652 (1973).

    Google Scholar 

  12. Kammer, G. M., Unnaue, E. R.: Accessory cell requirement in the proliferative response of T lymphocytes to hemocyanin. Clin. Immunol. Immunopathol.15, 434–443 (1980).

    Google Scholar 

  13. Kino, Y., Hayashi, Y., Hayashida, I., Mori, R.: Dissemination of herpes simplex virus in nude mice after intracutaneous inoculation and effect of antibody on the course of infection. J. gen. Virol.63, 475–479 (1982).

    Google Scholar 

  14. Larsen, H. S., Feigo, M., Horohov, D. W., Moore, R. N., Rouse, B. T.: Role of T-lymphocyte subsets in recovery from herpes simplex virus infection. J. Virol.50, 56–59 (1984).

    Google Scholar 

  15. Larsen, H. S., Russel, R. G., Rouse, B. T.: Recovery from lethal herpes simplex type 1 infection is mediated by cytotoxic T lymphocytes. Infect. Immun.41, 197–204 (1983).

    Google Scholar 

  16. Mori, R., Tasaki, G., Kimura, G., Takeya, K.: Depression of aquired resistance against herpes simplex virus infection in neonatally thymectomized mice. Arch. Gesamte Virusforsch.21, 459–462 (1967).

    Google Scholar 

  17. Nagafuchi, S., Hayashida, I., Higa, K., Wada, T., Mori, R.: Role of Lyt-1 positive immune T cells in recovery from herpes simplex virus infection in mice. Microbiol. Immunol.26, 359–362 (1982).

    Google Scholar 

  18. Nagafuchi, S., Oda, H., Mori, R., Taniguchi, T.: Mechanisms of aquired resistance to herpes simplex virus infection as studied in nude mice. J. gen. Virol.44, 715–723 (1979).

    Google Scholar 

  19. Nash, A. A., Gell, P. G. H.: Membrane phenotype of murine effector and suppressor T cells involved in delayed hypersensitivity and protective immunity to herpes simplex virus. Cell. Immunol.75, 348–355 (1981).

    Google Scholar 

  20. Nash, A. A., Quariety-Papafio, R., Wildy, P.: Cell-mediated immunity in herpes simplex virus infected mice: Functional analysis of lymph node cells during periods of acute and latent infection with reference to cytotoxic and memory cells. J. gen. Virol.19, 309–317 (1980).

    Google Scholar 

  21. Oakes, J. E.: Role of cell-mediated immunity in the resistance of mice to subcutaneous herpes simplex infection. Infect. Immun.12, 166–172 (1975).

    Google Scholar 

  22. Oakes, J. E., Rector, J. T., Lausch, R. N.: Lyt 1+ cells participate in recovery from ocular herpes simplex virus type 1 infection. Invest. Opthalmol. Vis. Sci.25, 188–194 (1984).

    Google Scholar 

  23. Schmid, D. S., Larsen, H. S., Rouse, B. T.: Role of I a antigen expression and secretory function of accessory cells in the induction of cytotoxic T lymphocyte responses against herpes simplex virus. Infect. Immun.37, 1138–1147 (1982).

    Google Scholar 

  24. Simmons, S. A., Nash, A. A.: Zosteriform spread of herpes simplex virus as a model of recrudescence and its use to investigate the role of immune cells in prevention of recurrent disease. J. Virol.52, 816–821 (1984).

    Google Scholar 

  25. Stingl, G., Gazze-Stingl, L. A., Aberer, W., Wolff, K.: Antigen presentation by murine Langerhans cells and its alteration by ultraviolet B light. J. Immunol.127, 1707–1713 (1981).

    Google Scholar 

  26. Streilein, J. W., Bergstresser, P. R.: Langerhans cells: Antigen presenting cells of the epidermis. Immunobiology168, 285–300 (1984).

    Google Scholar 

  27. Wolff, K., Stingl, G.: The Langerhans cell. J. Invest. Dermatol.80, 17–21 (1983).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Virology, School of Medicine, Kyushu University, Fukuoka, Japan

    S. Yasumoto, N. Okabe & R. Mori

Authors
  1. S. Yasumoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. Okabe
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Mori
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

With 1 Figure

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yasumoto, S., Okabe, N. & Mori, R. Role of epidermal Langerhans cells in resistance to herpes simplex virus infection. Archives of Virology 90, 261–271 (1986). https://doi.org/10.1007/BF01317375

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01317375

Keywords

Search

Navigation