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Nucleotide sequence comparisons of turnip yellow mosaic virus isolates from Australia and Europe

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Summary

The genomic sequences of four isolates of turnip yellow mosaic virus (TYMV-Cd) from Australia, and three TYMV-1 (type) and three TYMV-2 (cauliflower) isolates from Europe were compared by cDNA-RNA hybridization tests, by analysis of the fragments produced from cDNA-RNA hybrids by restriction endonuclease treatment, and by determining the 3′ terminal nucleotide sequences of their coat protein mRNAs. All three methods showed only slight differences (ca. 1%) between the mRNA sequences of different TYMV-1 and TYMV-Cd isolates, and did not distinguish between those groups of isolates. By contrast, the nucleotide sequences of TYMV-2 isolates differed from those of the other TYMVs by ca. 5% (sequence analysis) to 11% (restriction fragment analysis).

Published biogeographic evidence has indicated that the TYMV-Cd and TYMV-1 populations probably separated more than 12,000 years ago. This implies that these TYMV genomes have changed at a rate of, at most, 1% in 10,000 years.

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References

  1. Blok J, Air GM (1980) Comparative nucleotide sequences at the 3′ end of the neuraminidase gene from eleven influenza type A viruses. Virology 107: 50–60

    Google Scholar 

  2. Blok J, Air GM (1982) Sequence variation at the 3′ end of the neuraminidase gene from 39 influenza type A viruses. Virology 121: 211–229

    Google Scholar 

  3. Blok J, Henchal EA, Gorman BM (1984) Comparison of dengue viruses and some other flaviviruses by cDNA-RNA hybridization analysis and detection of a close relationship between dengue virus serotype 2 and Edge Hill virus. J Gen Virol 65: 2173–2181

    Google Scholar 

  4. Blok J (1985) Genetic relationships of the dengue virus serotypes. J Gen Virol 66: 1323–1325

    Google Scholar 

  5. Blok J, Gibbs AJ, Mackenzie A (1987) The classification of tymoviruses by cDNA-RNA hybridization and other measures of relatedness. Arch Virol 96: 225–240

    Google Scholar 

  6. Both GW, Air GM (1979) Nucleotide sequence coding for the N-terminal region of the matrix protein of influenza virus. Eur J Biochem 96: 363–372

    Google Scholar 

  7. Bouley JP, Briand JP, Geneveaux M, Pinck M, Witz J (1976) The structure of eggplant mosaic virus: evidence for the presence of low molecular weight RNA in top component. Virology 69: 775–781

    Google Scholar 

  8. Broadbent L, Heathcote GD (1985) Properties and host range of turnip crinkle, rosette and yellow mosaic viruses. Ann Appl Biol 46: 585–592

    Google Scholar 

  9. Dale J, Gibbs A (1976) Kennedya yellow mosaic virus: another tymovirus. Aust J Biol Sci 29: 397–403

    Google Scholar 

  10. Demeure M, Kummert J, Semal J (1979) Study of the products of reverse transcription of the RNA of turnip yellow mosaic virus primed by oligo dG6. Parasitica 35: 165–178

    Google Scholar 

  11. Faragher SG, Hutchison CA, Dalgarno L (1985) Analysis of Ross River virus genomic RNA usingHae III digests of single-stranded cDNA to infected-cell RNA and virion RNA. Virology 141: 248–256

    Google Scholar 

  12. Faragher SG, Marshall ID, Dalgarno L (1985) Ross River virus genetic variants in Australia and the Pacific islands. Aust J Exp Biol Sci 663: 473–488

    Google Scholar 

  13. Geneveaux M, Pinck M, Duranton HM (1976) Amino acid accepting structures in tymovirus RNAs. Ann Microbiol (Inst Pasteur) 127 A: 47–53

    Google Scholar 

  14. Gibbs A, Fenner F (1984) Methods for comparing sequence data such as restriction endonuclease maps or nucleotide sequences of viral nucleic acid molecules. J Virol Methods 9: 317–324

    Google Scholar 

  15. Gibbs AJ, Blok J, Coates DJ, Guy PL, Mackenzie A, Pigram N (1986) Turnip yellow mosaic virus in an endemic Australian alpineCardamine. In: Barlow BA (ed) Flora and fauna of alpine Australasia: ages and origins. CSIRO, Australia, pp 289–299

    Google Scholar 

  16. Gibbs AJ, Hecht-Poinar E, Woods RD, McKee RK (1966) Some properties of three related viruses: Andean potato latent, dulcamara mottle and ononis yellow mosaic. J Gen Microbiol 44: 177–193

    Google Scholar 

  17. Giege R, Briand JP, Mengual R, Ebel JP, Hirth L (1978) Valylation of the two RNA components of turnip-yellow mosaic virus and specificity of the tRNA aminoacylation reaction. Eur J Biochem 84: 251–256

    Google Scholar 

  18. Gonda TJ, Symons RH (1978) The use of hybridization analysis with complementary DNA to determine the RNA sequence homology between strains of plant viruses: its application to several strains of cucumoviruses. Virology 88: 361–370

    Google Scholar 

  19. Gower JC (1966) Some properties of latent root and vector methods used in multivariate analysis. Biometrika 53: 337–346

    Google Scholar 

  20. Guilley H, Briand JP (1978) Nucleotide sequence of turnip yellow mosaic virus coat protein mRNA. Cell 15: 735–744

    Google Scholar 

  21. Guy P, Gibbs A (1981) A tymovirus ofCardamine sp. from alpine Australia. Aust Plant Pathol 10: 12–13

    Google Scholar 

  22. Guy PL, Gibbs AJ (1985) Further studies on turnip yellow mosaic tymovirus isolates from an endemic AustralianCardamine. Plant Pathol 34: 532–544

    Google Scholar 

  23. Hewson HJ (1982) Flora of Australia.Brassicacae, vol. 8. Australian Government Publishing Service, Canberra, p 317

    Google Scholar 

  24. Klein C, Fritsch C, Briand JP, Richards KE, Jonard G, Hirth L (1976) Physical and functional heterogeneity in TYMV RNA: evidence for the existence of an independent messenger coding for coat protein. Nucleic Acids Res 3: 3043–3061

    Google Scholar 

  25. Lobigs M, Weir RC, Dalgarno L (1986) Genetic analysis of Kunjin virus isolated usingHae III andTaq I restriction digests of single-stranded cDNA to virion RNA. Aust J Exp Biol Med Sci 64: 185–196

    Google Scholar 

  26. Matthews REF (1980) Turnip yellow mosaic virus. CMI/AAB Descr Plant Viruses No. 230

  27. Molloy PL, Symons RH (1980) Cleavage of DNA. RNA hybrids by Type II restriction enzymes. Nucleic Acids Res 8: 2939–2946

    Google Scholar 

  28. Palukaitis P, Symons RH (1980) Nucleotide sequence homology of thirteen tobamovirus RNAs as determined by hybridization analysis with complementary DNA. Virology 107: 354–361

    Google Scholar 

  29. Paul HL, Gibbs AJ, Wittman-Leibold B (1980) The relationships of certain tymoviruses assessed from the amino acid composition of their coat proteins. Intervirology 13: 99–109

    Google Scholar 

  30. Peacock AC, Dingman CW (1968) Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels. Biochemistry 1: 668–674

    Google Scholar 

  31. Pleij CWA, Neeleman A, van Vloten-Doting L, Bosch L (1976) Translation of turnip yellow mosaic virus RNAin vitro: a closed and an open coat protein cistron. Proc Natl Acad Sci USA 73: 4437–4441

    Google Scholar 

  32. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467

    Google Scholar 

  33. Sano H, Feix G (1976) Terminal riboadenylate transferase fromEscherichia coli. Eur J Biochem 71: 577–583

    Google Scholar 

  34. Sippel AE (1973) Purification and characterization of adenosine triphosphate: ribonucleic acid adenyltransferase fromEscherichia coli. Eur J Biochem 37: 31–40

    Google Scholar 

  35. Symons RH, Rees MW, Short MN, Markham R (1963) Relationship between the ribonucleic acid and protein of some plant viruses. J Mol Biol 6: 1–5

    Google Scholar 

  36. Upton WB (1977) Estimation of DNA sequence divergence from comparison of restriction endonuclease digests. Nucleic Acids Res 4: 1257–1265

    Google Scholar 

  37. Van Belkum A, Bingkun J, Rietveld K, Pleij CWA, Bosch L (1987) Structural similarities among valine-accepting tRNA-like structures in tymoviral RNAs and elongator tRNAs. Biochemistry 26: 1144–1151

    Google Scholar 

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Author notes

  1. J. Blok

    Present address: Sir Albert Sakzewski Virus Research Laboratory, Royal Children's Hospital, Herston Road, 4029, Queensland, Australia

  2. P. Guy

    Present address: Tasmanian Department of Agriculture, Box 192B G.P.O., 7000, Hobart, Tasmania, Australia

Authors and Affiliations

  1. Research School of Biological Sciences, Australian National University, Canberra, A.C.T., Australia

    J. Blok, A. Mackenzie, P. Guy & A. Gibbs

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  1. J. Blok
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  2. A. Mackenzie
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  3. P. Guy
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  4. A. Gibbs
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Blok, J., Mackenzie, A., Guy, P. et al. Nucleotide sequence comparisons of turnip yellow mosaic virus isolates from Australia and Europe. Archives of Virology 97, 283–295 (1987). https://doi.org/10.1007/BF01314427

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  • DOI: https://doi.org/10.1007/BF01314427

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