Summary
Metronidazole inhibits cell division in gametophytes of the fernAnemia phyllitidis without changing morphogenetic patterns. Simultaneously the sensitivity of the prothallia against gibberellins which substitute for the natural sexual pheromone “antheridiogen A”, is increased.
The inhibition of cell division is accompanied by a loss of chlorophyll and by severe changes in the ultra-structure of the chloroplasts.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Chien, Y. W., 1976: Electrochemistry of Drug Action 1. Electroreduction of Ferredoxins. J. Pharm. Sci.65, 1471–1476.
Edwards, D. I., Schoolar, A. I., 1971: Inhibition of Sugar Synthesis and Potentiation of Chlorophyll Degradation in Sugar Cane Leaf Tissue by Metronidazole. Z. Pflanzenphysiol.64, 73–76.
—,Mathison, G. E., Platt, D. J., 1974: Metronidazole — an Antimicrobial Drug which Inhibits Photosynthesis. Z. Pflanzenphysiol.71, 424–427.
Eisbrenner, G., Bothe, H., 1979: Modes of Electron Transfer from Molecular Hydrogen inAnabaena cylindrica. Arch. Microbiol.123, 37–45.
Gosh, A. K., 1980: Study of the Effect of Metronidazole on Nuclear Constituent. Curr. Sci.49, 317–318.
Ings, R. M. J., McFadzean, J. A., Ormerod, W. E., 1974: The Mode of Action of Metronidazole inTrichomonas vaginalis and other Microorganisms. Biochem. Pharmacol.23, 1421–1429.
Lindmark, D. G., Müller, M., 1976: Antitrichononad Action, Mutagenity, and Reduction of Metronidazole and other Nitroimidazoles. Antimicrobial Agents and Chemotherapy10, 476–482.
Müller, M., Lindmark, D. G., 1976: Uptake of Metronidazole and Its Effect on Viability in Trichomonads andEntamoeba invadens Under Anaerobic and Aerobic Conditions. Antimicrobial Agents and Chemotherapy9, 696–700.
Näf, U., 1979: Antheridiogens and Antheridial Development. In: The Experimental Biology of Ferns (A. F. Dyer, ed.), pp. 435–470. London-New York-San Francisco: Academic Press.
O'Brien, R. W., Morris, J. G., 1972: Effects of Metronidazole on Hydrogen Production byClostridium acetobutylicum. Arch. Microbiol.84, 225–233.
Reynolds, E. S., 1963: The Use of Lead Citrate at High pH as an Electron Opaque Stain in Electron Microscopy, J. Cell Biol.17, 208–212.
Sagromsky, H., 1975: Chlorophyllbestimmungen mittels eines Aceton-Diäthyläther-Methanol-Petroläther-Gemisches. Kulturpflanze23, 217–221.
Sanvordeker, D. R., Chien, Y. W., Lin, T. K., Lambert, J. H., 1975: Binding of Metronidazole and Its Derivatives to Plasma Proteins: An Assessment of Drug Binding Phenomenon. J. Pharm. Sci.64, 1797–1803.
Schmidt, G. W., Matlin, K. S., Chua, N.-H., 1977: A Rapid Procedure for Selective Enrichement of Photosynthetic Electron Transport Mutants. Proc. natl. Acad. Sci.74, 610–614.
Schraudolf, H., 1962: Die Wirkung von Phytohormonen auf Keimung und Entwicklung von Farnprothallien. I. Auslösung der Antheridienbildung und Dunkelkeimung bei Schizaeaceen durch Gibberellinsäure. Biol. Zentralbl.81, 731–740.
—, 1966: Wirkung von Phytohormonen auf Keimung und Entwicklung von Farnprothallien. II. Analyse der Wechselbeziehung zwischen Gibberellinkonzentration, Antheridienbildung und physiologischem Alter der Prothalliumzellen vonAnemia phyllitidis. Planta68, 335–352.
—, 1977: Effects of 5-Bromo-2-Deoxyuridine and 2-Aminopurine on Antheridium Differentiation inAnemia phyllitidis. Experientia33, 1161–1162.
—,Šonka, J., 1979: Effects of 5-Bromodeoxyuridine on Chloroplast Structure in Gametophytes ofAnemia phyllitidis L. Sw. Europ. J. Cell Biol.19, 135–138.
Šonka, J., Schraudolf, H., 1979: Incorporation of 5-Bromo-2-Deoxyuridine into Chloroplast DNA ofAnemia phyllitidis. Z. Naturforsch.34 c, 449–451.
Suzuki, T., Uchiyama, M., 1975: Photoreduction of Parathion by Spinach Chloroplasts. Chem. Pharm. Bull.23, 2175–2178.
Templeton, R., 1977: Metabolism and Pharmacokinetics of Metronidazole: A Review. In: Metronidazole (Finegold, S. M., ed.), pp.28–49, Excerpta Medica.
Tetley, R. M., Bishop, N. I., 1979: The Differential Action of Metronidazole on Nitrogen Fixation, Hydrogen Metabolism, Photosynthesis and Respiration inAnabaena andScenedesmus. Biochim. biophys. Acta546, 43–53.
Walles, B., 1967 a: The Homozygous and Heterozygous Effects of an Aurea Mutation on the Plastid Development in Spruce. (Picea abies (L.) Karst.). Studia Forestalia Suecia60, 1–10.
—, 1967 b: Use of Biochemical Mutants in Analysis of Chloroplast Morphogenesis. In: Biochemistry of Chloroplasts (Goodwin, T. W., ed.), pp. 633–653. London-New York: Academic Press.
Wardmann, P., Clarke, E. D., 1976: Oxygen Inhibition of Nitroreductase — Electron Transfer from Nitro-Radical-Anions to Oxygen. Biochem. Biophys. Res. Comm.69, 942–949.
Willson, R. L., Cramp, W. A., Ings, R. M. J., 1974: Metronidazole (‘Flagyl’): Mechanisms of Radiosensitization. Int. J. Radiat. Biol.26, 557–569.
Author information
Authors and Affiliations
Additional information
Metronidazole has been kindly supplied by Bayer AG, Leverkusen. The author has to thank Mrs. C.Guha, Mrs.Mayer and Mr. K.Russ for excellent technical assistance, as well as Mrs. S.Krug for checking the English version of the manuscript.
Rights and permissions
About this article
Cite this article
Schraudolf, H. Effects of metronidazole on growth, chloroplast structure and differentiation in gametophytes ofAnemia phyllitidis L. Sw. . Protoplasma 113, 144–149 (1982). https://doi.org/10.1007/BF01282004
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01282004