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Spot inoculation of soybean roots withRhizobium japonicum

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Summary

A method has been developed to inoculate soybean roots in the zone of susceptible root cells with nanoliter droplets containing the symbiont,Rhizobium japonicum. The position of inoculation is marked by means of ion exchange beads which adhere to the root surface. InoculatedR. japonicum cells from a nanoliter droplet spread over an area of approximately 35 host epidermal cells and have a high probability (∼ 80%) of inducing nodule formation at exactly the point of inoculation. The spot inoculation method is highly efficient in terms of the number of bacteria required to achieve a given probability of nodule formation. The method greatly facilitates ultrastructural analysis of the infection process and is useful for certain physiological studies of infection.

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References

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Authors and Affiliations

  1. Charles F. Kettering Research Laboratory, Yellow Springs, Ohio

    B. G. Turgeon & W. D. Bauer

Authors
  1. B. G. Turgeon
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  2. W. D. Bauer
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Turgeon, B.G., Bauer, W.D. Spot inoculation of soybean roots withRhizobium japonicum . Protoplasma 115, 122–128 (1983). https://doi.org/10.1007/BF01279804

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  • DOI: https://doi.org/10.1007/BF01279804

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