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Absorption of dietary triacylglycerol by lipolysis and lipid resynthesis in the mesenteron of larvalAeshna cyanea (insecta, odonata)

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Summary

Voluntary uptake of triolein, margarine, and lipid-rich natural food (Tubifex) by fasting dragonfly larvae (Aeshna cyanea) led to heavy accumulations of lipid absorption droplets in the enterocytes within 2 days, while subsequent lipid clearance of the midgut epithelium took several weeks depending on the ingested lipid load. No endocytotic lipid uptake was observed after application of a molecular-dispersed fat dye. The smallest lipid droplets first appeared in the subapical groundplasm of the enterocytes and showed a reversible increase in size on their way towards the base. Lipid droplets were also observed at appropriate intervals after oral administration of oleic acid, after feeding margarine in the cold, and after injection of triolein into the isolated midgut.

Comparative biochemical analysis after triolein feeding evidenced release of lipase and hydrolytic liberation of FA from TG in the midgut lumen, as well as time-dependent accumulations of TG in the midgut epithelium and of DG in the hemolymph.

Oral injection of [14C] oleic acid was followed by its rapid absorption into the midgut epithelium, where it was utilized for the synthesis of MG and esterification to DG and TG. Discharge of radioactive lipid into the hemolymph occurred in the form of FA and DG, while the rectal fat body showed approximately equal labeling of the FA, DG, and TG fractions.

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Abbreviations

AG:

acylglycerol

DG:

diacylglycerol

ER:

endoplasmic reticulum

FA:

fatty acid

MG:

monoacylglycerol

TG:

triacylglycerol

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Authors and Affiliations

  1. Institute of Cytology, University of Bonn, Ulrich-Haberland-Strasse 61a, D-5300, Bonn, Federal Republic of Germany

    H. Komnick & J. Kukulies

  2. Institute of Applied Zoology, University of Bonn, Federal Republic of Germany

    J. Bongers & W. Fischer

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  1. H. Komnick
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  2. J. Kukulies
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  3. J. Bongers
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  4. W. Fischer
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Dedicated to Prof. Dr. Dr. R.Lehmensick, Bonn, in honor of his 85th birthday.

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Komnick, H., Kukulies, J., Bongers, J. et al. Absorption of dietary triacylglycerol by lipolysis and lipid resynthesis in the mesenteron of larvalAeshna cyanea (insecta, odonata) . Protoplasma 123, 57–69 (1984). https://doi.org/10.1007/BF01283182

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