Summary
Tomato cotyledon explants, cultured in vitro in the presence of sucrose, were subjected to different hormonal treatments to establish whether the induction of different organogenic programmes could be correlated with differences in starch accumulation and protein electrophoretic pattern. The cytohistological changes in explants over the first 15 days of culture were studied by light and electron microscopy. It was found that starch accumulation occurs under all conditions, though varying in duration and amount. Over the first 2 days of culture the protein electrophoretic pattern changes in a similar way under all conditions, while after 7 days changes take place which are probably related to the different developmental programmes induced by the treatments.
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Abbreviations
- BOA:
-
benzisoxazole-3-acetic acid
- 2,4-D:
-
2,4 dichloro-phenoxyacetic acid
- DTT:
-
dithiothreitol
- GA3 :
-
gibberellic acid
- HF:
-
hormone-free
- LM:
-
light microscopy
- LSB:
-
Laemmli sample buffer
- PAGE:
-
polyacrylamide gel electrophoresis
- PMSF:
-
phenylmethylsulfonylfluoride
- TEM:
-
transmission electron microscopy
- ZR:
-
zeatine riboside
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Branca, C., Torelli, A., Fermi, P. et al. Early phases in in vitro culture of tomato cotyledons: Starch accumulation and protein pattern in relation to the hormonal treatment. Protoplasma 182, 59–64 (1994). https://doi.org/10.1007/BF01403689
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DOI: https://doi.org/10.1007/BF01403689