Summary
The effects of brefeldin A (BFA) on the structure of the Golgi apparatus, the nuclear envelope, and the endoplasmic reticulum (ER), and on the thiamine pyrophosphatase (TPPase) activity in these organelles were examined in a green alga,Scenedesmus acutus, to obtain evidence for the existence of a retrograde transport from the Golgi apparatus to the ER via the nuclear envelope. InScenedesmus, Golgi bodies are situated close to the nuclear envelope throughout the cell cycle and receive the transition vesicles not directly from the ER, but from the nuclear envelope. BFA induced the disassembly of Golgi bodies and an increase in the ER cisternae at the trans-side of decomposed Golgi bodies in interphase cells and multinuclear cells before septum formation. The accumulated ER cisternae connected to the nuclear envelope at one part. TPPase activity was detected in all cisternae of Golgi bodies, but not in the nuclear envelope or the ER in nontreated cells. On the contrary, in BFA-treated cells, TPPase activity was detected in the nuclear envelope and the ER in addition to the decomposed Golgi bodies. When septum-forming cells were treated with BFA, the disassembly of Golgi bodies was less than that in interphase cells, and TPPase activity was detected in the Golgi cisternae but not in the nuclear envelope or the ER. These results suggest mat BFA blocks the anterograde transport from the nuclear envelope to the Golgi bodies but does not block the retrograde transport from the Golgi bodies to the nuclear envelope in interphase and multinuclear cells.
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Abbreviations
- BFA:
-
brefeldin A
- ER:
-
endoplasmic reticulum
- TPPase:
-
thiamine pyrophosphatase
References
Bracker CE, Morré DJ, Grove SN (1996) Structure, differentiation, and multiplication of Golgi apparatus in fungal hyphae. Protoplasma 194: 250–274
Donaldson JG, Finazzi D, Klausner RD (1992) Brefeldin A inhibits Golgi membrane-catalysed exchange of guanine nucleotide onto ARF protein. Nature 360: 350–352
Driouich A, Zhang GF, Staehelin LA (1993) Effect of brefeldin A on the structure of the Golgi apparatus and on the synthesis and secretion of proteins and polysaccharides in sycamore maple (Acer pseudoplatanus) suspension-cultured cells. Plant Physiol 101: 1363–1373
Fujiwara T, Oda K, Yokota S, Takatsuki A, Ikehara Y (1988) Brefeldin A causes disassembly of the Golgi complex and accumulation of secretory proteins in the endoplasmic reticulum. J Biol Chem 263: 18545–18552
Helms JB, Rothman JE (1992) Inhibition by brefeldin A of a Golgi membrane enzyme that catalyses exchange of guanine nucleotide bound to ARF. Nature 360: 352–354
Johnson UG, Porter KR (1968) Fine structure of cell division inChlamydomonas reinhardi: basal bodies and microtubules. J Cell Biol 38: 403–425
Kimura S, Yamada M, Igaue I, Mitsui T (1993) Structure and function of the Golgi complex in rice cells: characterization of Golgi membrane glycoproteins. Plant Cell Physiol 34: 855–863
Klausner RD, Donaldson JG, Lippincott-Schwartz J (1992) Brefeldin A: insights into the control of membrane traffic and organelle structure. J Cell Biol 116: 1071–1080
Kreis TM, Lowe M, Pepperkok R (1995) COPs regulating membrane traffic. Annu Rev Cell Dev Biol 11: 677–706
Lippincott-Schwartz J, Yuan LC, Bonifacino JS, Klausner RD (1989) Rapid redistribution of Golgi proteins into the ER in cells treated with brefeldin A: evidence for membrane cycling from Golgi to ER. Cell 56: 801–813
—, Donaldson, JG, Schweizer A, Berger EG, Hauri HP, Yuan LC, Klausner RD (1990) Microtubule-dependent retrograde transport of proteins into the ER in the presence of brefeldin A suggests an ER recycling pathway. Cell 60: 821–836
Marsh ME (1994) Polyanion-mediated mineralization-assembly and reorganization of acidic polysaccharides in the Golgi system of a coccolithophorid alga during mineral deposition. Protoplasma 177: 108–122
Misumi Y, Misumi Y, Miki K, Takatsuki A, Tamura G, Ikehara Y (1986) Novel blockade by brefeldin A of intracellular transport of secretory proteins in cultured rat hepatocytes. J Biol Chem 261: 11398–11403
Mollenhauer HH, Morre DJ (1976) Cytochalasin B, but not colchicine, inhibits migration of secretory vesicles in root tips of maize. Protoplasma 87: 39–48
Noguchi T (1976) Phosphatase activities and osmium reduction in cell organelles ofMicrasterias americana. Protoplasma 87: 163–178
Pelham HRB (1991) Multiple targets for Brefeldin A. Cell 67: 449–451
Robinson DG (1980) Dictyosome-endoplasmic reticulum associations in higher plant cells? A serial-section analysis. Eur J Cell Biol 23: 22–36
— (1993) Brefeldin A: a tool for plant cell biologists? Bot Acta 106: 107–109
Rutten TLM, Knuiman B (1993) Brefeldin A effects on tobacco pollen tubes. Eur J Cell Biol 61: 247–255
Satiat-Jeunemaitre B, Hawes C (1992a) Reversible dissociation of the plant Golgi apparatus by brefeldin A. Biol Cell 74: 325–328
— — (1992b) Redistribution of a Golgi glycoprotein in plant cells treated with brefeldin A. J Cell Sci 103: 1153–1166
— — (1993) Insights into the secretory pathway and vesicular transport in plant cells. Biol Cell 79: 7–15
— — (1994) G.A.T.T. (a general agreement on traffic and transport) and brefeldin A in plant cells. Plant Cell 6: 463–467
— —, Hawes C (1996) Golgi-membrane dynamics are cytoskeleton dependent: a study on Golgi stack movement induced by brefeldin A. Protoplasma 191: 21–33
Salomon S, Meindl U (1996) Brefeldin A induces reversible dissociation of the Golgi apparatus in the green algaMicrasterias. Protoplasma 194: 231–242
Ueda K, Noguchi T (1986) Structure of dictyosomes inScenedesmus acuminatus observed by the freeze-substitution method. J Cell Sci 82: 217–222
Yasuhara H, Sonobe S, Shibaoka H (1995) Effects of brefeldin A on the formation of the cell plate in tobacco BY-2 cells. Eur J Cell Biol 66: 274–281
Watanabe MM, Satake KN (1991) NIES-Collection: list of strains, 3rd edn. The National Institute for Environmental Studies, Tukuba
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Noguchi, T., Watanabe, H. & Suzuki, R. Effects of brefeldin A on the Golgi apparatus, the nuclear envelope, and the endoplasmic reticulum in a green alga,Scenedesmus acutus . Protoplasma 201, 202–212 (1998). https://doi.org/10.1007/BF01287416
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DOI: https://doi.org/10.1007/BF01287416