Summary
The ultrastructure of fructose-supplemented and unsupplemented nitrogen-fixing (fix +) and nonfixing (fix −)Plectonema boryanum UTEX 581 cells was examined by transmission electron microscopy. The most prominent structural differences included the arrangement and morphology of the thylakoids and alterations in the appearance of the interthylakoidal spaces. These ultrastructural differences, together with other observations such as glycogen content and presence of nitrogenase (using acetylene reduction assay and immunogold localization), readily distinguished nonfixingP. boryanum from nitrogen-fixing cells.
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References
Allen MM (1968) Simple conditions for growth of unicellular blue-green algae on plates. J Phycol 4: 1–4
—, Smith AJ (1969) Nitrogen chlorosis in blue-green algae. Arch Mikrobiol 69: 114–120
Barnum SR, Gendel SM (1987) Heterotrophic growth of nine strains of filamentous cyanobacteria. Iowa State J Res 62: 147–159
Bergman B, Lindblad P, Rai AN (1986) Nitrogenase in free-living and symbiotic cyanobacteria: immunoelectron microscopic localization. FEMS Microbiol Lett 35: 75–78
Chao L, Bowen CC (1971) Purification and properties of glycogen isolated from a blue-green alga,Nostoc muscorum. J Bacteriol 105: 331–338
De Vasconcelos L, Fay P (1974) Nitrogen metabolism and ultrastructure inAnabaena cylindrica. I. The effect of nitrogen starvation. Arch Microbiol 96: 271–279
Ernst A, Kirchenlohr H, Diez J, Böger P (1984) Glycogen content and nitrogenase activity inAnabaena variabilis. Arch Microbiol 140: 120–125
Fay P (1965) Heterotrophy and nitrogen fixation inChlorogloea fritschii. J Gen Microbiol 39: 11–20
—, Lang NJ (1971) The heterocyst of blue-green algae. I. Ultrastructural integrity after isolation. Proc R Soc Lond [Biol] 178: 185–192
Giesy RM (1964) A light and electron microscope study of interlamellar polyglucoside bodies inOscillatoria chalybia. Am J Bot 51: 388–396
Jensen TE (1985) Cell inclusions in the cyanobacteria. Arch Hydrobiol 71 [Suppl 1–2]: 33–73
—, Bowen CC (1970) Cytology of blue-green algae. II. Unusual inclusions in the cytoplasm. Cytologia 35: 132–152
Lang NJ (1965) Electron microscopic study of heterocyst development inAnabaena azollae Strasburger. J Phycol 1: 127–154
—, Rae PMM (1967) Structure in a blue-green alga resembling prolamellar bodies. Protoplasma 64: 67–74
Lehmann M, Wöber G (1976) Accumulation, mobilization, and turnover of glycogen in the blue-green bacteriumAnacystis nidulans. Arch Microbiol 11: 93–97
Lockau W, Peterson RB, Wolk CP, Burris RH (1978) Modes of reduction of nitrogenase in heterocysts isolated fromAnabaena species. Biochim Biophys Acta 502: 298–308
Miller LS, Holt SC (1977) Effect of carbon dioxide on pigment and membrane content inSynechococcus lividus. Arch Microbiol 115: 185–198
Murry MA, Hallenbeck PC, Benemann JR (1984) Immunochemical evidence that nitrogenase is restricted to the heterocysts inAnabaena cylindrica. Arch Microbiol 137: 194–199
Peat A, Whitton BA (1967) Environmental effects on the structure of the blue-green alga,Chlorogloea fritschii. Arch Microbiol 57: 155–160
Raboy B, Padan E, Shilo M (1976) Heterotrophic capabilities ofPlectonema boryanum. Arch Mikrobiol 110: 77–85
Rippka R, Waterbury JB (1977) The synthesis of nitrogenase by non-heterocystous cyanobacteria. FEMS Microbiol Lett 2: 83–86
Ris H, Singh RN (1961) Electron microscope studies on blue-green algae. J Biophys Biochem Cytol 9: 63–80
Singh PK (1973) Nitrogen fixation by the unicellular blue-green algaAphanotheca. Arch Mikrobiol 92: 59–62
Starr RC (1978) The culture collection of algae at the University of Texas at Austin. J Phycol 14 [Suppl]: 47–100
Stevens SE, Balkwill DL, Paone DAM (1981) The effects of nitrogen limitation on the ultrastructure of the cyanobacteriumAgmenellum quadruplicatum. Arch Microbiol 130: 204–212
Stewart WDP, Lex M (1970) Nitrogenase activity in the blue-green algaPlectonema boryanum strain 594. Arch Mikrobiol 73: 250–260
—, Fitzgerald GP, Burris RH (1967) In situ studies on nitrogen fixation using the acetylene reduction technique. Proc Natl Acad Sci USA 58: 2071–2078
Vaughn KC (1987) Two immunological approaches to the detection of ribulose, 1,5-biphosphate carboxylase in guard cell chloroplasts. Plant Physiol 84: 188–196
von Hahn H (1961) The specificity of osmium tetroxide as fixative and reagent in histochemistry. Conn Univ Biol Bull 3: 1–5
Weber M, Wöber G (1975) The fine structure of the branched α-glucan from the blue-green alga,Anacystis nidulans: comparison with other bacterial glycogens and phytoglycogen. Carbohydr Res 39: 295–302
Wyatt JT, Silvey JKG (1969) Nitrogen fixation byGleocapsa. Science 165: 908–909
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Smoker, J.A., Owen, H.A., Lehnen, L.P. et al. Ultrastructure of the nitrogen-fixing, filamentous, nonheterocystous cyanobacterium,Plectonema boryanum . Protoplasma 152, 130–135 (1989). https://doi.org/10.1007/BF01323072
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DOI: https://doi.org/10.1007/BF01323072