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Ultrastructural responses of tobacco pollen tubes to heat shock

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Summary

The effect of elevated temperatures on semivivo growth and ultrastructure of tobacco pollen tubes was investigated. Tube growth was decreased by about 50% at 35 °C, independent of the duration of treatment, and at 40 °C and above there was no growth of tubes. Heat treatment caused ultrastructural changes like accumulation of membranous materials, concentric stacking of rough endoplasmic reticulum, reduction in vesicle production by dictyosomes, increase in the fenestrated regions of the Golgi cisternae, swelling of mitochondrial saccules and increase in the electron density of the mitochondrial matrix. Furthermore, the dictyosomes of the treated tubes showed significant increase in the number of cisternae from 30 to 45 °C. The temperature induced changes were persistant at least for 24 h in 35 °C grown pollen tubes. The possible reasons for the tube growth inhibition are discussed on the basis of the ultrastructural alterations caused by elevated temperatures.

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Abbreviations

HSPs:

heat shock proteins

SD:

standard deviation

ER:

endoplasmic reticulum

ATP:

adenosine triphosphate

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Author notes

  1. M. K. Kandasamy

    Present address: Section of Plant Biology, Plant Science Building, Cornell University, 14853, Ithaca, NY, USA

Authors and Affiliations

  1. Institut für Allgemeine Botanik, Universität Hamburg, Hamburg

    M. K. Kandasamy & U. Kristen

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  1. M. K. Kandasamy
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  2. U. Kristen
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Kandasamy, M.K., Kristen, U. Ultrastructural responses of tobacco pollen tubes to heat shock. Protoplasma 153, 104–110 (1989). https://doi.org/10.1007/BF01322470

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  • DOI: https://doi.org/10.1007/BF01322470

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