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Localization of α-amylase isozymes within the endomembrane system of barley aleurone

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Summary

The localization of α-amylase (EC 3.2.1.1) in barley (Hordeum vulgare L. cv Himalaya) aleurone protoplasts was studied using electron microscope immunocytochemistry. Antibodies were raised against total barley α-amylase, i.e., α-amylase containing both highisoelectric point (high-pI) and low-pI isoforms, as well as against purified high- and low-pI isoforms. All antibodies localized α-amylase to the endoplasmic reticulum (ER) and Golgi apparatus (GApp) of the aleurone cell, and various controls showed that the labeling was specific for α-amylase. Labeling of protein bodies and spherosomes, which are the most abundant organelles in this cell, was very low. There was no evidence that α-amylase isoforms were differentially distributed within different compartments of the endomembrane system. Rather, both high- and low-pI isoforms showed the same pattern of distribution in ER and in the cis, medial, and transregions of the GApp. We conclude that in the Himalaya cultivar of barley, all isoforms of α-amylase are transported to the plasma membrane via the GApp.

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Abbreviations

ER:

endoplasmic reticulum

GA3 :

gibberellic acid

GApp:

Golgi apparatus

PBS:

phosphate buffered saline

PCR:

partially coated reticulum

PM:

plasma membrane

TBS:

Tris buffered saline

TGN:

trans-Golgi network

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Authors and Affiliations

  1. Plant Physiology Institute, University of Göttingen, Göttingen

    Irmgard Zingen-Sell, S. Hillmer & D. G. Robinson

  2. Department of Plant Biology, University of California, Berkeley, California

    R. L. Jones

Authors
  1. Irmgard Zingen-Sell
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  2. S. Hillmer
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  3. D. G. Robinson
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  4. R. L. Jones
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Zingen-Sell, I., Hillmer, S., Robinson, D.G. et al. Localization of α-amylase isozymes within the endomembrane system of barley aleurone. Protoplasma 154, 16–24 (1990). https://doi.org/10.1007/BF01349531

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  • DOI: https://doi.org/10.1007/BF01349531

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