Summary
The claim that osmium-containing deposits which lack lead are frequently and incorrectly interpreted as enzymatic reaction products in lead precipitation techniques for ATPase localization in plants is without foundation. Proper controls clearly demonstrate the enzymatic origin of membrane-located deposits and the presence of lead is confirmed by analytical electron microscopy.
Similar content being viewed by others
References
Brentwood, B. J., Cronshaw, J., 1978: Cytochemical localization of adenosine triphosphatase in the phloem ofPisum sativum and its relation to the function of transfer cells. Planta (Berl.)140, 111–120.
Browning, A. J., Hall, J. L., Baker, D. A., 1980: Cytochemical localization of ATPase activity in phloem tissues ofRicinus communis. Protoplasma104, 55–65.
Gilder, J., Cronshaw, J., 1974: A biochemical and cytochemical study of adenosine triphosphatase activity in the phloem ofNicotiana tabacum. J. Cell Biol.60, 221–235.
Hall, J. L., 1969: A histochemical study of adenosine triphosphatase and other nucleotide phosphatases in young root tips. Planta (Berl.)89, 254–265.
—, 1971: Cytochemical localization of ATPase activity in plant root cells. J. Micros.93, 219–225.
—,Al-Azzawi, M. J., Fielding, J. L., 1979: Microscopic cytochemistry in enzyme localization and development. In: Regulation of enzyme synthesis and activity in higher plants (Smith, H., ed.). London: Academic Press.
Hall, J. L., Dymott, A., 1980: Localization and properties of adenosine triphosphatase in wheat coleoptiles. In: Plant membrane transport: current conceptual issues, pp. 517–518 (Spanswick, R. M., Lucas, W. J., Dainty, J., eds.). Amsterdam: Elsevier/North Holland.
Malone, C. P., Burke, J. J., Hanson, J. B., 1977: Histochemical evidence for the occurrence of oligomycin-sensitive plasmalemma ATPase in corn roots. Plant Physiol.60, 916–922.
Pope, D. G., Black, M., 1972: The effect of indole-3-acetic acid on coleoptile extension growth in the absence of protein synthesis. Planta (Berl.)102, 26–36.
Robards, A. W., Kidwai, P., 1969: Cytochemical localization of phosphatase in differentiating secondary vascular cells. Planta (Berl.)87, 227–238.
Sauter, J. J., 1977: Electron microscopical localization of adenosine triphosphatase and β-glycerophosphatase in sieve cells ofPinus nigra var.austriaca (Hoess) Badoux. Z. Pflanzenphysiol.81, 438–458.
Sexton, T., Hall, J. L., 1978: Enzyme cytochemistry. In: Electron microscopy and cytochemistry of plant cells (Hall, J. L., ed.). Amsterdam: Elsevier/North Holland.
Taylor, A. R. D., Hall, J. L., 1978: Fine structure and cytochemical properties of tobacco leaf protoplasts and comparison with the source tissue. Protoplasma96, 113–126.
Tice, L. W., 1969: Lead-adenosine triphosphate complexes in adenosine triphosphatase histochemistry. J. Histochem. Cytochem.17, 85–94.
Van Steveninck, R. F. M., 1979: The verification of cytochemical tests for ATPase activity in plant cells using X-ray microanalysis. Protoplasma99, 211–220.
-Iwahori, S., 1977: Do plasmodesmata possess ATPase activity. In: Echanges ioniques transmembranaires chez les végétaux, pp. 499–506 (Thellier, M.,Monnier, A.,Demarty, M.,Dainty, J., eds.). Colloque CNRS No. 258.
Winter-Sluiter, E., Lauchli, A., Kramer, D., 1977: Cytochemical localization of K+-stimulated adenosine triphosphatase activity in xylem parenchyma cells of barley roots. Plant Physiol.60, 923–927.
Yapa, P. A. J., Spanner, D. C., 1974: Localization of adenosine triphosphatase activity in mature sieve elements ofTetragonia. Planta (Berl.)117, 321–328.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hall, J.L., Browning, A.J. & Harvey, D.M.R. The validity of the lead precipitation technique for the localization of ATPase activity in plant cells. Protoplasma 104, 193–200 (1980). https://doi.org/10.1007/BF01279767
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01279767