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Influence ofRhizobium leguminosarum biovartrifolii host specific nodulation genes on the ontogeny of clover nodulation

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Summary

The infection of white clover seedlings byRhizobium strains with different host range properties was assessed using various microscopic techniques. Several wild-type andRhizobium leguminosarum biovarvicias hybrid strains containing definedR. l. bv.trifolii host range genes were used. The morphological changes in the root tissue of uninoculated and rhizobia inoculated white clovers were identified and compared. In particular, changes were observed in the induction of inner cortical cell division, alterations to nodule development and lateral root formation. The responses of the infected roots and the types of structures formed support the hypothesis that lateral roots and nodules may be physiologically homologous structures. To establish a normal pattern of nodulation on white clover roots, both sets of known host specific nodulation genes (operonsnod FERL andnod MNX) ofR. l. bv.trifolii were required. However, some nodule development occurred when only thenod FERL genes were present in the hybrid strain.

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Authors and Affiliations

  1. Plant-Microbe Interaction Group, Institute of Advanced Studies, Reserach School of Biological Sciences, Australian National University, P.O. Box 475, 2601, Canberra City, ACT, Australia

    J. Mclver, M. A. Djordjevic, J. J. Weinman & B. G. Rolfe

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  1. J. Mclver
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  2. M. A. Djordjevic
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  3. J. J. Weinman
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  4. B. G. Rolfe
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Mclver, J., Djordjevic, M.A., Weinman, J.J. et al. Influence ofRhizobium leguminosarum biovartrifolii host specific nodulation genes on the ontogeny of clover nodulation. Protoplasma 172, 166–179 (1993). https://doi.org/10.1007/BF01379374

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  • DOI: https://doi.org/10.1007/BF01379374

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