Summary
Scanning electron micrographs of barley protoplasts were compared using various preparatory techniques. Numerous features were observed which turned out to be artifactual characteristics of the processing procedure used in collecting and dehydrating the samples. The most successful technique gave protoplasts which presumably maintained their natural structural integrity, as judged by retention of sphericity and absence of holes in the plasma membrane. The relative numbers of fragmented protoplasts and cellular organelles was also greatly reduced.
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Hughes, B.G., White, F.G. & Smith, M.A. Scanning electron microscopy of barley protoplasts. Protoplasma 90, 399–405 (1976). https://doi.org/10.1007/BF01275690
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DOI: https://doi.org/10.1007/BF01275690