Summary
Diurnal changes in the concentration of cAMP in white clover were investigated biochemically and histochemically. Biochemical assays showed that a) the concentration of cAMP in healthy leaves was consistently higher than in CYMV-diseased leaves under the same light condition and b) the concentration of cAMP in clover leaves exhibited a diurnal cycle. The diurnal changes in cAMP concentration were present in both healthy and CYMV-diseased clover leaves. Histochemical localization tests indicated that the adenylate cyclase activity could be semi quantilated as more dense reaction products were present in the membrane systems of healthy than CYMV-diseased leaves. The histochemical results were consistent with the biochemical data.
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Tu, J.C. Biochemical and histochemical investigation of diurnal variation in cAMP concentration and adenylate cyclase activity in white dutch clover. Protoplasma 99, 139–146 (1979). https://doi.org/10.1007/BF01274075
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DOI: https://doi.org/10.1007/BF01274075