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Ion permeation properties of a cloned human 5-HT3 receptor transiently expressed in HEK 293 cells

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Summary

Human 5-HT3 receptors expressed in HEK 293 cells were studied using patch-clamp techniques. The permeability ratios of cations to Na+ were Li+, 1.16; K+, 1.04; Rb+, 1.11; Cs+ 1.11; NMDG+, 0.04; Ca2+, 0.49, and Mg2+, 0.37. The permeability sequence of the alkali metal cations was Li+ > Rb+ = Cs+ > K+ > Na+. Increased external concentrations of Ca2+ or Mg2+ decreased 5-HT-induced currents at all potentials tested in a voltage-independent manner. The single-channel conductance of human 5-HT3 receptors measured by fluctuation analysis of whole-cell currents was 790 ± 100fS. Differences in the basic properties of 5-HT3 receptors between species may explain interspecies differences in pharmacological properties.

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Authors and Affiliations

  1. Molecular Medicine Laboratories, Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co., Ltd., 21 Miyukigaoka Tsukuba, 305, Ibaraki, Japan

    Shinobu Mochizuki, A. Miyake & K. Furuichi

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  1. Shinobu Mochizuki
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  2. A. Miyake
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  3. K. Furuichi
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Mochizuki, S., Miyake, A. & Furuichi, K. Ion permeation properties of a cloned human 5-HT3 receptor transiently expressed in HEK 293 cells. Amino Acids 17, 243–255 (1999). https://doi.org/10.1007/BF01366923

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  • DOI: https://doi.org/10.1007/BF01366923

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