Summary
Rat hearts were exposed and injured locally by direct cryoapplication. The hearts were examined 2, 4, 20 and 48 hours, 7 and 12 days later.
In the first 48 hours mast cell and polymorphonuclear (mainly eosinophilic) granulocyte infiltration was followed by proliferation of mononuclear phagocytic and fibroblastic cells and new capillaries. The underlying muscle showed freezethaw-rigor damage, segmental intramyofiber necrosis, phagocytosis and focal regeneration.
After 7 days numerous myofibroblasts appeared and they were intimately related to myofibers undergoing degeneration and repair.
After 12 days there was increased fibrosis while mesothelial covering cells of the epicardium and myofibers returned to normal.
It is suggested that amines released from mast cells are involved in the formation of myofibroblasts.
Zusammenfassung
Nach operativer Freilegung wurden beim Herzen von Ratten durch direkte Vereisung eine örtliche Schädigung gesetzt. Die Herzen wurden 2, 4, 20 und 48 Stunden sowie 7 und 12 Tage später untersucht.
In den ersten 48 Stunden trat eine Infiltration mit Mastzellen und polymorphkernigen Granulozyten (hauptsächlich Eosinophilen) auf, gefolgt von einer Proliferation mononuklearer Phagozyten, fibroplastischen Zellen und neuer Kapillaren. Die benachbarten Muskelpartien zeigten eine Schädigung im Sinne eines Einfrier-Auftau-Rigors, segmentaler Muskelfaser-Nekrose, Phagozytose und fokaler Regeneration.
Nach 7 Tagen erschienen zahlreiche Myofibroblasten, die enge Beziehungen zeigten zu Muskelfasern, die der Degeneration und Regeneration unterlagen.
Nach 12 Tagen war eine verstärkte Bindegewebsbildung zu verzeichnen mit mesothelialen Deckzellen des Epikardiums, die Muskelfasern normalisierten sich.
Es wird angenommen, daß Amine, die von den Mastzellen freigesetzt werden, an der Bildung der Myofibroblasten beteiligt sind.
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This work was supported by the Muscular Dystrophy Association of America.
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Yarom, R., Havivi, Y. & Heling, I. Myofibroblasts in subepicardium after local cold injury. Basic Res Cardiol 74, 250–261 (1979). https://doi.org/10.1007/BF01907742
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DOI: https://doi.org/10.1007/BF01907742