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Effects of α-tocopherol and its acetate on the hydrolytic activity of phospholipase D in egg yolk phosphatidylcholine bilayers

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Abstract

Effects of α-tocopherol (Toc) and α-tocopherol acetate (TocA) on the hydrolysis activity of phospholipase D (fromStreptomyces chromofuscus) were studied in small unilamellar vesicles (SUV) of egg yolk phosphatidylcholine (PC). Choline produced in the reaction was monitored by use of a choline oxidase — oxygen electrode. Addition of 18 mol% Toc into SUV (2 mM PC) brought about a twofold rate of choline production. On the other hand, the effect of 18 mol% TocA in SUV was very small. The apparent maximum velocity,V max(app), increased by addition of Toc in SUV. The apparent Michaelis constant,K m(app), was unchanged by addition of Toc and TocA in SUV. The Toc and TocA molecules did not have significant effects when PC was solubilized in the micelles of heptaethylene glycol dodecyl ether. The effects of Toc and TocA are, therefore, not due to specific ones on the enzyme itself, but rather upon the bilayer-organization of the substrate. Measurements of spreading pressure showed complete miscibility of PC and Toc, and limited mutual solubility of PC and TocA, suggesting stronger attractive interactions between Toc and PC than those between TocA and PC in the bilayers.

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  1. I. Yamamoto

    Present address: Faculty of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, 606-01, Kyoto, Japan

Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, 606-01, Kyoto, Japan

    T. Mazumi, Y. Asai, T. Handa & K. Miyajima

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  1. I. Yamamoto
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  4. T. Handa
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Yamamoto, I., Mazumi, T., Asai, Y. et al. Effects of α-tocopherol and its acetate on the hydrolytic activity of phospholipase D in egg yolk phosphatidylcholine bilayers. Colloid Polym Sci 272, 598–603 (1994). https://doi.org/10.1007/BF00653227

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  • DOI: https://doi.org/10.1007/BF00653227

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