Abstract
The sympathetic innervation of human and dog livers was examined by immunohistochemical localization of neuron-specific enolase to visualize the total complement of hepatic nerves and the localization of two enzymes involved in catecholamine synthesis, tyrosine hydroxylase and dihydroxyphenylalanine decarboxylase, to visualize sympathetic nerves. Similar results were obtained for both man and dog. About 60% of the non-myelinated axons supplying the hepatic parenchyma, and virtually all those supplying the vasculature, appeared to be sympathetic. The pattern of dihydroxyphenylalanine decarboxylase immunoreactivity was compatible with innervation of the intrahepatic hepatic arteries and portal veins by dopaminergic as well as by noradrenergic sympathetic nerves. By contrast, there was no evidence for a dopaminergic component in the parenchymal sympathetic innervation.
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References
Kuntz A.The Autonomic Nervous System. Philadelphia: Lea & Febiger, 1953; 245–250.
Sutherland SD. An evaluation of the cholinesterase techniques in the study of the intrinsic innervation of the liver.J Anat 1964;98: 321–328.
Amenta FD, Cavallotti C, Ferrante F, Tonelli F. Cholinergic nerves in the human liver.Histochem J 1981;13: 419–424.
Reilly FD, McCuskey PA, McCuskey RS. Intrahepatic distribution of nerves in the rat.Anat Rec 1978;191: 55–68.
Satler JJ, Predan S, Brzin M. The activity and cytochemical localisation of acetylcholinesterase and butyrylcholinesterase in the rat liver.Histocbemistry 1974;39: 65–70.
Reilly FD, McCuskey RS, Cilento EV. Hepatic microvascular regulatory mechanisms.Microvasc Res 1981;21: 103–106.
Forssmann WG, Ito S. Hepatocyte innervation in primates.J Cell Biol 1977;74: 299–313.
Metz W, Forssmann WG. Innervation of the liver in guinea-pig and rat.Anat Embryol 1980;160: 239–252.
Kyösola K, Penttila O, Ihamaki T, Varis K, Salaspuro M. Adrenergic innervation of the human liver. A fluorescence histochemical analysis of clinical liver biopsy specimens.Scand J Gastroenterol 1985;20: 254–256.
Moghimzadeh E, Nobin A, Rosengren E. Adrenergic nerves and receptors in the liver.Brain Res Bull 1982;9: 709–714.
Lautt WW. Afferent and efferent neural roles in liver function.Prog Neurobiol 1983;21: 323–348.
Hirsch LJ, Ayabe T, Glick G. Direct effects of various catecholamines on liver circulation in dogs.Am J Pbysiol 1976;230: 1394–1399.
Matsumoto M, Miyazaki T, Mizukami S, Nakajima T, Hori T. Effects of dopamine, dobutamine and phenylephrine on the hepatic circulation and hepatic metabolism during halothane and non-halothane anaesthesia.Masui 1984;33: 837–843.
Shimazu M, Aoki A, Mita S, Miyakita K. The effects of dopamine on liver circulation in man.Nihon Sbokakibyo 1986;83: 1153–1160.
Richardson PDI, Withrington PG. Responses of the canine hepatic arterial and portal venous vascular beds to dopamine.Eur J Pharmacol 1978;48: 337–349.
Oka T, Iigima K, Kohei H, Matsumai T, Yorozu S, Mizuguchi T. Effects of dopamine on hepatic circulation in dogs.Masui 1986;35: 1207–1211.
Roytblat L, Gelman S, Bradley EL, Henderson T, Parks D. Dopamine and hepatic oxygen supply-demand relationship.Can J Physiol Pbarmacol 1990;68: 1165–1169.
Bell C. Peripheral dopaminergic nerves.Pharmacol Ther 1989;44: 157–179.
Hökfelt T, Fuxe K, Goldstein M. Applications of immunohistochemistry to studies on monoamine cell systems with special reference to nervous tissues.Ann NY Acad Sci 1975;254: 407–432.
Jaeger CB, Ruggiero DA, Albert VR, Park DH, Job TH, Reis DJ. Aromatic L-amino acid decarboxylase in the rat brain: immunocytochemical localization in neurons of the brain stem.Neuroscience 1984;11: 691–713.
Harris T, Muller B, Cotton RGH, Borri Voltattomi C, Bell C. Dopaminergic and noradrenergic sympathetic nerves of the dog have different dopa decarboxylase activities.Neurosci Lett 1986;65: 155–160.
Ferguson M, Ryan GB, Bell C. The innervation of the renal cortex in the dog.Cell Tissue Res 1988;253: 539–546.
Bell C, Bhathal PS, Mann R, Ryan GB. Evidence that dopaminergic sympathetic axons supply the medullary arterioles of human kidney.Histocbemistry 1989;91: 361–364.
Shimazu T. Reciprocal innervation of the liver: its significance in metabolic control.Adv Metab Disord 1983;10: 355–384.
Sawchenko PE, Friedman MI. Sensory functions of the liver—a review.Am J Pbysiol 1979;236: R5-R20.
Mann R, Bell C. Neuronal metabolism and dopa decarboxylase immunoreactivity in terminal noradrenergic sympathetic axons of rat.J Histocbem Cytochem 1991;39: 663–668.
Mawe GM, Gershon MD. Structure, afferent innervation and transmitter content of ganglia of the guinea-pig gallbladder: relationship to the enteric nervous system.J Comp Neurol 1989;283: 374–390.
Furness JB, Costa M.The Enteric Nervous System. Edinburgh: Churchill Livingstone London, 1987; 71.
Mann R, Borri Voltattorni C, Bell C. Extrinsic denervation elevates neuronal aromatic L-amino acid decarboxylase immunoreactivity in rat small intestine.Histocbemistry 1989;92: 219–223.
Mann R. Catecholaminergic innervation of the gallbladder in dog and man.Proc Aust Pbysiol Pbarmacol Soc 1990;21: 132P.
Burns AM, Hill SA, Park GR. The effects of dopamine and dopexamine on isolated preparations of human blood vessels.J A uton Pharmacol 1990;10 (Suppl 1): s129.
Burt AD, Tiniakos D, MacSween RNM, Griffiths MR, Wisse E, Polak JM. Localization of adrenergic and neuropeptide tyrosine-containing nerves in the mammalian liver.Hepatology 1989;9: 839–845.
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Mann, R., Bhathal, P.S. & Bell, C. Sympathetic innervation of the liver in man and dog: An immunohistochemical study. Clinical Autonomic Research 1, 141–145 (1991). https://doi.org/10.1007/BF01826211
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DOI: https://doi.org/10.1007/BF01826211