Summary
A specific and sensitive procedure for simultaneous determination of 5,5-diphenylhydantoin, 5-(4-hydroxyphenyl)-5-phenylhydantoin, 5-(3,4-dihydroxyphenyl)-5-phenylhydantoin and 5-(3-methoxy-4-hydroxyphenyl)-5-phenylhydantoin in biological specimens is described. The method involves formation of butylated or trideuteromethylated derivatives of the drug and its metabolites and analysis by gas chromatography. The lower detectable concentration of these compounds per ml of body fluids or g of wet tissue is either 1 μg or 20 ng, depending on the type of detector used, flame ionization or selected-ion monitoring. Data on the urinary elimination of 5,5-diphenylhydantoin and hydroxylated metabolites in male rats following a single intraperitoneal injection of the drug (25 mg/kg) are also reported.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
J. Laidlaw, A. Richens (Editors), A text-book of Epilepsy, Churchill, Edinburgh, 1976.
S. I. Johannessen, P. L. Morselli, C. E. Pippenger, A. Richens, S. Schmidt, H. Meinardi (Editors). Antiepileptic Therapy: Advances in Drug Monitoring, Raven Press, New York, 1980.
A. J. Glazko, Drug Metab. Dispos.1, 711 (1973).
W. A. Dill, A. Kazenko, L. M. Wolf, A. J. Glazko, J. Pharmacol. Exp. Ther.118, 270 (1956).
W. A. Dill, A. J. Glazko, Clin. Chem.18, 675 (1972).
D. H. Sandberg, G. L. Resnick, C. Z. Bacallao, Anal. Chem.40, 736 (1968).
G. Grimmer, J. Jacob, H. Schafer, Arzneim. Forsch.19, 1287 (1969).
J. McGee, Anal. Chem.42, 421 (1970).
H. J. Kupferberg, Clin. Chem. Acta29, 282 (1970).
P. M. Kabra, B. E. Stafford, L. J. Marton, Clin. Chem.23, 1284 (1977).
M. G. Horning, C. M. Butler, J. Nowlin, K. Lertratanangkoon, T. E. Zion, P. Kellaway, R. M. Hill, inA. Rickens, F. P. Woodford (Editors), Anticonvulsivant Drugs and Enzyme Induction, Elsevier, Amsterdam, 1976, p. 61.
M. G. Horning, C. M. Butler, K. Lertratanangkoon, R. M. Hill, T. E. Zion, P. Kellaway, inP. Kellaway, I. Petersen (Editors), Quantitative Analytic Studies in Epilepsy, Raven Press, New York, 1976, p. 95.
M. Arboix, C. Pantarotto, in 39th International Congress of Pharmaceutical Sciences, Brighton, September 3–7, 1979, abstract no. 100.
G. Lhoëst, J. H. Poupaert, M. Claesen, Eur. J. Mass Spectrom. Biochem. Med. Environ. Res.1, 57 (1980).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Arboix, M., Pantarotto, C. Determination of 5,5-diphenylhydantoin and its major metabolites in biological specimens by gas chromatography and selected ion-monitoring. Chromatographia 15, 509–513 (1982). https://doi.org/10.1007/BF02260285
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02260285