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Separation of single-stranded oligonucleotides on polyvinyl alcohol gel column

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Summary

Separation mechanisms for single-stranded oligodeoxyribo-or oligoribonucleic acid fragments were explored on an Asahipak polyvinyl alcohol gel column (GS-320) by use of sequential isomers of such molecules. Substrates having different base numbers were found to be separated by size-exclusion chromatography while those having the same numbers with different base sequences were isolated by use of the reversed-phase mode. By using those dual modes, a limit for the separation of the samples was found to arise because one mode shifted the peaks of the substrates in the sense opposite to the shift resulting from the other mode and it was found that when substrates had less than nine bases, the solutes eluted separately.

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Author notes

  1. T. Fukui

    Present address: Research Laboratory, Dainippon Pharmaceutical Company, Enoki 33-94, Suita, 564, Osaka, Japan

Authors and Affiliations

  1. Department of Polymer Science and Engineering, Faculty of Textile Science, Kyoto Institute of Technology, Matsugasaki Sakyo-ku, 606, Kyoto, Japan

    H. Wada & H. Ozaki

  2. Department of Chemistry, Faculty of Science, Kyoto University, Kitashirakawa, Sakyo-ku, 606, Kyoto, Japan

    H. Hatano

  3. Faculty of Pharmaceutical Sciences, Osaka University, Yamadaoka, Suita, 565, Osaka, Japan

    T. Fukui

  4. Technology and Development Department, Asahi Chemical Ind. Co. Ltd., 1-3-2 Yakoo, Kawasaki-ku, 210, Kawasaki, Japan

    K. Noguchi & Y. Yanagihara

  5. Research Laboratory, Dainippon Pharmaceutical Company, Enoki 33-94, Suita, 564, Osaka, Japan

    K. Makino & T. Takeuchi

Authors
  1. K. Makino
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  2. H. Wada
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  3. H. Ozaki
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  4. T. Takeuchi
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  5. H. Hatano
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  6. T. Fukui
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  7. K. Noguchi
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  8. Y. Yanagihara
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Makino, K., Wada, H., Ozaki, H. et al. Separation of single-stranded oligonucleotides on polyvinyl alcohol gel column. Chromatographia 20, 713–716 (1985). https://doi.org/10.1007/BF02282928

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  • DOI: https://doi.org/10.1007/BF02282928

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