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A reversed-phase liquid chromatographic columns for the analysis of single-stranded oligo-DNAs

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Summary

A reversed-phase liquid chromatographic (RPLC) column, with which large oligodeoxynucleotides (oligo-DNAs) can be separated in well-resolved peaks, has been developed. The column contains only specially selected silica bonded with monochlorodimethyloctadecylsilane. The carbon content of the column packing is the predominant factor to achieve good results. With this column several mixtures containing different large oligo-DNAs could be separated with excellent resolution.

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Authors and Affiliations

  1. Department of Polymer Science and Engineering, Faculty of Textile Science, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, 606, Kyoto, Japan

    K. Makino, H. Ozaki, H. Imaishi & T. Takeuchi

  2. Faculty of Pharmaceutical Science, Osaka University, Yamadaoka, 565, Suita, Osaka, Japan

    T. Fukui

  3. Department of Chemistry, Faculty of Science, Kyoto University, Kitashirakawa, Sakyou-ku, 606, Kyoto, Japan

    H. Hatano

Authors
  1. K. Makino
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  2. H. Ozaki
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  3. H. Imaishi
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  4. T. Takeuchi
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  5. T. Fukui
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  6. H. Hatano
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Additional information

Dedicated to Professor Morio Ikehara of Osaka University, on the occasion of his retirement in March 1986.

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Makino, K., Ozaki, H., Imaishi, H. et al. A reversed-phase liquid chromatographic columns for the analysis of single-stranded oligo-DNAs. Chromatographia 23, 247–253 (1987). https://doi.org/10.1007/BF02311773

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  • DOI: https://doi.org/10.1007/BF02311773

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