Summary
The influence of the eluent composition, ion-pair concentration, and temperature on the fractionation of chromosomal proteins by reversed-phase high performance liquid chromatography (HPLC) has been investigated. Based on this examination methods for the troublesome separation of histones H1o/H1b, H2A/H4, and H5.1/H5.2 have been developed.
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Dedicated to Prof. Dr. Dr. h. c. Erika Cremer on the occasion of her 90th birthday.
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Lindner, H., Helliger, W. Effects of eluent composition, ion-pair reagent and temperature on the separation of histones by high performance liquid chromatography. Chromatographia 30, 518–522 (1990). https://doi.org/10.1007/BF02269797
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DOI: https://doi.org/10.1007/BF02269797