Skip to main content
SpringerLink
Log in

Liquid chromatographic separation of monoamino analogues of dideoxyadenosine and 9-(dideoxy-β-D-lyxo-pentofuranosyl)adenine from adenine

  • Originals
  • Published:
Chromatographia Aims and scope Submit manuscript

Summary

A liquid chromatographic method that can separate each of the monoamino analogues of dideoxyadenosine and 9-(-dideoxy-β-D-lyxo-pentofuranosyl)adenine from their main degradation product, adenine, is described. The influence of the pH of the mobile phase, the type and concentration of the organic modifier and the concentration of the silanol masking agent and the buffer have been investigated. Several reversed phases were examined. Samples were finally analyzed on a 10 μm Spherisorb ODS1 column (250 mm×4.6 mm I.D.) using acetonitrile −0.2 M potassium phosphate buffer pH 6.0–0.2 M tetramethylammonium phosphate pH 6.0-water (5∶5∶0.5∶89.5, v/v) as the mobile phase. The interactions between the solute and the stationary phase are discussed in view of the basic properties of the compounds under study.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. P. Herdewijn, J. Balzarini, R. Pauwels, G. Janssen, A. Van Aerschot, E. De Clercq. Nucleosides and Nucleotides8, 1231 (1989).

    Google Scholar 

  2. C. M. Riley, J. Chromatogr.531, 295 (1990).

    PubMed  Google Scholar 

  3. P. R. Brown, “HPLC in Nucleic Acid Research”, Marcel Dekker, Inc., New York, 1984; p. 21.

    Google Scholar 

  4. European Pharmacopoeia, 2nd Ed., Maisonneuve, Sainte-Ruffine, France, 1987; V. 6.20.4.

  5. J. Clauwaert, J. Stockx, Z. Naturforsch.23b, 25 (1968).

    Google Scholar 

  6. G. Thoithi, A. Van Schepdael, R. Busson, P. Herdewijn, E. Roets, J. Hoogmartens, in preparation.

  7. L. R. Snyder, J. L. Glajch, J. J. Kirkland, “Practical HPLC Method Development”, John Wiley and Sons, New York, 1988; p. 128.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratorium voor Farmaceutische Chemie, Instituut voor Farmaceutische Wetenschappen, Katholieke Universiteit Leuven, Van Evenstraat 4, 3000, Leuven, Belgium

    G. Thoithi, A. Van Schepdael, P. Herdewijn, E. Roets & J. Hoogmartens

Authors
  1. G. Thoithi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Van Schepdael
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. Herdewijn
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. Roets
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. Hoogmartens
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Thoithi, G., Van Schepdael, A., Herdewijn, P. et al. Liquid chromatographic separation of monoamino analogues of dideoxyadenosine and 9-(dideoxy-β-D-lyxo-pentofuranosyl)adenine from adenine. Chromatographia 35, 451–454 (1993). https://doi.org/10.1007/BF02278601

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02278601

Key Words

Search

Navigation