Summary
A new highly sensitive high-performance liquid chromatographic (HPLC) procedure for determination of EGIS-9933 (a newly developed anxiolytic compound) in rat plasma is described. A gradient, elution method with UV detection at 270 nm has been developed using a mobile phase of a mixture of A: methanol:acetonitrile 1:9 and B:0.5% triethilamine in water, the pH of B was adjusted to 3 with phosphoric acid. Solid phase extraction (SPE) was used for the sample preparation. The calibration was linear in the 10–10000 ng mL−1 concentration range. The limit of quantification was 10 ng mL−1. The bioanalytical method was validated according to internationally accepted criteria for biological samples.
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References
I. Jakóczi, D. Bózsing, I. Simonek, I. Gacsályi, G. Szénási, É. Schmidt, A. Kovács, A. Bilkei Gorzó, G. Blaskó, I. Gyertyán, G. Németh, Gy. Simig, K. Tihanyi, A. Egyed, Novel piperazinyl-alkyl-thio-pyrimidin derivatives, Pharmaceutical compositions containing the same and a process for the preparation of the novel compounds, Patent application No.: WO 97/16429 (1997).
P. Shah, K. K. Midha, S. Dighe, I. J. McGilveray, J. P. Skelly, A. Yacobi, T. Layloff, C. T. Viswanathan, C. E. Cook, R. D. McDowall, K. A. Pittman, S. Spector, Eur. J. Drug Metab. Pharmacokin.16, 249 (1991).
R. Bucik, M. V. Doig, S. C. Jeal, G. S. Land, R. D. McDowall, J. Pharmaceut. Biomed. Anal.8, 629 (1990).
T. Karnes, S. G. Shin, V. P. Shah, Pharmaceutical Research8, 421 (1991).
P. Shah, K. K. Midha, S. Dighe, Pharmaceutical Research9, 588 (1991).
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Nemes, K.B., Szúnyog, J. & Klebovich, I. A new validated high-performance liquid chromatographic method for the determination of EGIS-9933 in rat plasma. Chromatographia 48, 158–162 (1998). https://doi.org/10.1007/BF02467535
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DOI: https://doi.org/10.1007/BF02467535