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Separation of carotenoid mixtures using centrifugal chromatography

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Summary

Centrifugal chromatography was used as a new method for the separation of carotenoid mixtures. Among others, a good separation resulted with α-carotene/β-carotene, α-cryptoxanthin/β-cryptoxanthin/rubixanthin, zeaxanthin/isozeaxanthin/lutein and with all β-carotene epoxides. Microparticulate silica and magnesium oxide were used as stationary phases, benzene with additions of petroleum ether or methanol as mobile phases. The development time was 2 to 6 minutes. The column could be charged with a maximum of 0.5 mg per pigment component.

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Authors and Affiliations

  1. Institute of Organic Chemistry, University of Berne, Berne, Switzerland

    H. Pfander & F. Haller

  2. Department of Vitamin and Nutritional Research, F. Hoffmann-La Roche & Co., Ltd., Basle, Switzerland

    F. J. Leuenberger

  3. Department of Agrochemistry, F. Hoffmann-La Roche & Co., Ltd., Basle, Switzerland

    H. Thommen

Authors
  1. H. Pfander
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  2. F. Haller
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  3. F. J. Leuenberger
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  4. H. Thommen
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Pfander, H., Haller, F., Leuenberger, F.J. et al. Separation of carotenoid mixtures using centrifugal chromatography. Chromatographia 9, 630–632 (1976). https://doi.org/10.1007/BF02290534

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  • DOI: https://doi.org/10.1007/BF02290534

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