Crystallization and preliminary X-ray diffraction study of the flavoenzyme 2,4-pentadienoyl-CoA reductase from Clostridium aminovalericum

The tetrameric flavoenzyme 2,4-pentadienoyl-CoA reductase has been crystallized from solutions containing polyethylene glycol as precipitant. The crystals grow in the monoclinic space group C2 with unit-cell dimensions a = 160.2, b = 120.2, c = 95.3 Å, β = 99.0°. The packing parameter V_M is 2.3 ų Da⁻¹ (Matthews parameter) for four monomers per asymmetric unit. Complete data sets to about 2.9 Å resolution have been collected.