Crystallization and preliminary X-ray studies of rhamnogalacturonase A from Aspergillus aculeatus

Recombinant rhamnogalacturonase A from Aspergillus aculeatus has been crystallized and X-ray diffraction data has been collected. Crystals were grown by the hanging-drop vapour-diffusion technique, under the conditions 10% PEG 8000, 0.05 M KH₂PO₄ and 0.1 M sodium acetate buffered at pH 3.5. The crystals diffract beyond 2.0 Å resolution and belong to one of the orthorhombic space groups I2₁2₁2₁ or I222, with the unit-cell parameters a = 62.9, b = 125.4 and c = 137.0 Å. There is one molecule in the asymmetric unit and a solvent content of approximately 54%. The enzyme is highly glycosylated corresponding to 5.9 kDa.