Isolation of photosystem II reaction center complex by affinity chromatography with the peripheral 33-kDa polypeptide as ligand

doi:10.1016/0014-5793(87)80424-6

FEBS Letters

Volume 224, Issue 1, 16 November 1987, Pages 71-74

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Abstract

We report a procedure for one-step isolation of the PS II reaction center complex from PS II particles by affinity chromatography with the peripheral 33-kDa polypeptide as ligand. The CaCl₂-washed PS II particles prepared from spinach chloroplasts were solubilized with n-octyl-β-d-glucopyranoside and subjected to affinity chromatography. The purified complex consisted of polypeptides with apparent molecular masses of 47, 43, 32, 30 and 8 kDa.

Keywords

Oxygen evolution

Photosystem II

Reaction center

Affinity chromatography

33 kDa polypeptide

Photosynthesis

Abbreviations

Chl

chlorophyll

DCIP

2,6-dichloro-indophenol

DPC

1,5-diphenylcarbazide

Mes

4-morpholineethanesulfonic acid

Mops

3-N-morpholinepropanesulfonic acid

octylglucoside

n-octyl-β-d-glucopyranoside

PAGE

polyacrylamide gel electrophoresis

photosystem