Elsevier

FEBS Letters

Volume 161, Issue 2, 19 September 1983, Pages 257-260
FEBS Letters

Essential protein factors for polyprenyl pyrophosphate synthetases: Separation of heptaprenyl pyrophosphate synthetase into two components

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Abstract

Heptaprenyl pyrophosphate synthetase from Bacillus subtilis was dissociated into two essential components, none of which had catalytic activity alone. The enzyme activity was restored when the two components were combined with each other. Both fractions, designated components I and II in the order of their elution from DEAE-Sephadex, appeared to be proteins of Mr 30 000. Component I was much more stable than component II which was easily destroyed by relatively mild heat treatment. Neither was inter-changeable with any of the essential components of hexaprenyl pyrophosphate synthetase of Micrococcus luteus B-P 26.

Keywords

Heptaprenyl pyrophosphate synthetase
Bacillus subtilis
Polyprenyl pyrophosphate
Prenyltransferase
Polyprenol

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1

Present address: Research Institute for Tuberculosis and Cancer, Tohoku University, Sendai 980, Japan