Elsevier

FEBS Letters

Volume 201, Issue 1, 26 May 1986, Pages 25-30
FEBS Letters

Research letters
A monoclonal antibody specific for snRNPs U1 and U2

https://doi.org/10.1016/0014-5793(86)80564-6Get rights and content
Under an Elsevier user license
open archive

Abstract

A monoclonal antibody (D-5) is described which selectively precipitates snRNPs U1 and U2. The antibody was derived from a mouse immunized with extracts from chick embryonic nuclei. By immunoblotting on either total proteins from purified snRNPs U1–U6, U2–U6 or U1 only, we could demonstrate that the monoclonal antibody cross-reacts with the U1 RNP specific polypeptide A and the U2 RNP specific polypeptide B'', thereby establishing that the two snRNP proteins share at least one epitope. D-5 precipitates snRNPs U1 and U2 from a variety of species, including man, chicken, mouse, rat kangaroo and Xenopus laevis. It will thus be a useful tool for studying structure function relationships of the two snRNP species in different cell systems.

Keywords

U snRNP
RNA processing
Splicing
Monoclonal antibody
Lupus erythematosus
Antigenic structure

Cited by (0)