Elsevier

FEBS Letters

Volume 306, Issue 1, 13 July 1992, Pages 80-84
FEBS Letters

A TIM barrel protein without enzymatic activity? Crystal-structure of narbonin at 1.8 Å resolution

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Abstract

The major protein component in seeds is storage protein. These have no known enzymatic activity and act to provide amino acids as a source of metabolites in the developing seedling. We report here the first three dimensional crystal structure of a seed storage globulin at high resolution. The molecule of the 2S globulin, narbonin, from Vicia narbonensis L. consists of an eight-stranded parallel α/gb barrel structure similar to that observed in triose phosphate isomerase (TIM). Narbonin is the first protein with this topology possessing no known enzymatic activity. Because of the lack of sequence information most of the primary structure was determined directly from the electron density.

Keywords

Seed storage protein
Vicia narbonensis
X-ray structure
TIM barrel
Primary structure

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