Reversed-phase ion-pair chromatographic method for the determination of nitroprusside in photolyzed solutions

https://doi.org/10.1016/S0003-2670(00)85791-8Get rights and content

Abstract

A rapid reversed-phase ion-pair chromatographic method for disodium pentacyanonitrosylferrate(II) (nitroprusside) and its photodegradation products nitrite, nitrate, hexacyanoferrate(II) and hexacyanoferrate(III) is described. For chromatography, phenyl-bonded pellicular silica gel (10 μm) was used with a mobile phase consisting of water (0.005 M tetrabutylammonium phosphate, 0.0011 M n-octylamine, 0.01 M potassium dihydrogenphosphate, pH 7.0) and methanol (0.005 M tetrabutylammonium phosphate, 0.0011 M n-octylamine) (65:35); the detector was set at 220 nm. In 5% (w/v) dextrose solutions, the calibration graph for nitroprusside was linear over the concentration range 10–120 μg ml−1. A qualitative explanation for the order of retention: hexacyanoferrate(II) < hexacyanoferrate(III) < nitroprusside is given. The method is suitable for the selective determination of nitroprusside in photolyzed infusion solutions (100 μg ml−1 in aqueous 5% dextrose) and gives an impression of the decomposition products formed.

References (23)

  • R.P. Mitra et al.

    J. Inorg. Nucl. Chem.

    (1963)
  • G.V. Buxton et al.

    Int. J. Radiat. Phys. Chem.

    (1969)
  • D.M. Baaske et al.

    J. Chromatogr.

    (1981)
  • G. Gritzner et al.

    J. Electroanal. Chem.

    (1976)
  • H.E. Toma

    Inorg. Chim. Acta

    (1975)
  • D.J. Kenney et al.

    J. Inorg. Nucl. Chem.

    (1961)
  • A. Goodman Gilman et al.
  • A. Goodman Gilman et al.
  • A. Overbeck

    Arch. Pharm. (Weinheim)

    (1853)
  • I.M. Kolthoff

    Z. Anal. Chem.

    (1923)
  • E. Justin-Mueller

    Bull. Soc. Chim. Fr.

    (1935)
  • Cited by (9)

    • Sodium Nitroprusside

      1986, Analytical Profiles of Drug Substances and Excipients
    View all citing articles on Scopus
    View full text