Elsevier

Journal of Chromatography A

Volume 160, Issue 1, 11 January 1978, Pages 155-168
Journal of Chromatography A

Quantitative gas chromatographic method for the analysis of aliphatic hydrocarbons, terpenes, fatty alcohols, fatty acids and sterols in tobacco

https://doi.org/10.1016/S0021-9673(00)91792-8Get rights and content

Abstract

A rapid and reproducible method is described for the analysis of the major tobacco lipids, including the C25C34 paraffinic hydrocarbons, neophytadiene, phytol, docosanol, squalene, α-tocopherol, β-amyrin, cycloartenol, 24-methylenecycloartanol, palmitic acid, stearic acid, the unsaturated C18 acids, cholesterol, stigmasterol, campesterol, sitosterol and solanesol. The method is based upon the short-column silicic acid chromatography of saponified lipids from a hexane extract of tobacco. The free lipids are separated into non-polar, polar and terpenoid fractions and quantitated by gas chromatography, with the use of internal standards. The applicability of the method has been demonstrated by the highly reproducigle analyses of a cigarette blend, conventional flue-cured tobaccos and two close-grown tobaccos. The method should also be applicable to analyses of lipids in other natural products, including foodstuffs.

References (11)

  • R.F. Severson et al.

    J. Chromatogr.

    (1977)
  • D.L. Davis

    Recent Advan. Tobacco Sci.

    (1976)
  • I. Schmeltz et al.
  • W.S. Schlotzhauer et al.

    J. Agr. Food Chem.

    (1976)
  • O.T. Chortyk et al.

    Beitr. Tabakforsch.

    (1975)
There are more references available in the full text version of this article.

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    The major sterols in tobacco leaves are cholesterol, campesterol, stigmasterol and β-sistosterol and the are present in amount from 0.1% to 0.3% of tobacco leaves dry weight (Leffingwell, 1999). Severson et al. (1978) developed tobacco sterols in different part of tobacco plant. They found that sterols were present in all tobacco plant parts but their level in the stalk and flowers was significantly lower.

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