High-performance liquid chromatography and capillary supercritical-fluid chromatography separation of vegetable carotenoids and carotenoid isomers
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Green Separation Techniques for -Omics Platforms: Supercritical Fluid Chromatography
2020, Comprehensive FoodomicsEffect of centrifugation speed on the measured equilibrium solubility of poorly water-soluble compounds in viscous solvents
2020, Journal of Drug Delivery Science and TechnologyCitation Excerpt :Amphotericin B was detected at a wavelength of 406 nm with a mobile phase consisting of methanol: 5 mM EDTA-Ca-2Na buffer (70:30, v/v) at a flow rate of 1 ml/min. β-carotene was detected at a wavelength of 461 nm with a mobile phase consisting of methanol: acetonitrile: chloroform (47:47:6, v/v) at a flow rate of 1.8 ml/min [10]. Curcumin was detected at a wavelength of 425 nm with a mobile phase of acetonitrile: 100 mM ammonium acetate buffer at pH 3.5 (45:55, v/v) at a flow rate of 1 ml/min [11].
Advances of supercritical fluid chromatography in lipid profiling
2019, Journal of Pharmaceutical AnalysisCitation Excerpt :Prenol lipids (PRs) mainly consist of fat-soluble vitamins (i.e. vitamins A, E and K), carotenoids and ubiquinones (i.e. coenzyme Q9). In previous research, cSFC-UV has demonstrated significant potential for the improvement of the separation of carotenoids and their isomers from carrots and tomatoes relative to HPLC-UV [50]. The technology of pSFC-MS has been used for the analysis of PRs in recent years.
Supercritical Fluid Chromatography
2017, The Application of Green Solvents in Separation ProcessesStatistical optimization of airlift photobioreactor for high concentration production of torularhodin pigment
2016, Biocatalysis and Agricultural BiotechnologyDetermination of carotenoids in microalgae using supercritical fluid extraction and chromatography
2012, Journal of Chromatography ACitation Excerpt :Hence more rapid routine analysis of carotenoids could be carried out using SFC and also in a sustainable way. Despite the advantages of SFC, a very few number of authors have investigated the possibilities to separate different carotenoids using SFC. Before packed columns became popular some attention was given to the separation of α and β-carotene isomers using capillary columns [9,10], and also applied to separate carotenoids in carrots and tomato extracts [11]. However, presently packed columns are used exclusively, although very little research has been conducted on applying this technique in order to separate carotenoids.