Studies on a new cross-axis coil planet centrifuge for performing counter-current chromatography: I. Design of the apparatus, retention of the stationary phase, and efficiency in the separation of proteins with polymer phase systems
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Cited by (52)
Relayed chromatography - Countercurrent chromatography in series with liquid chromatography for the separation of natural products
2022, Journal of Chromatography ACitation Excerpt :In recent years, CCC has arisen and provided natural product researchers with a novel separation method that has been widely applied to the isolation and purification of natural products [2–8]. Nevertheless, the lower theoretical plates of classic CCC result in an inferior separation efficiency [9–13], which affects CCC methods to be efficiently used in the preparative separation of structurally similar compounds with high chemical purity. Several novel methods have been developed to increase the CCC efficiency in recent years.
Scale-up protein separation on stainless steel wide bore toroidal columns in the type-J counter-current chromatography
2015, Journal of Chromatography ACitation Excerpt :With a portfolio of innovative CCC columns being explored [2,5–7], their scaling-up has been justified through increasing the column diameter [8], the same as HPLC. Up to now, positive results for protein separation in this area have been obtained in the following situations, (a) use of cross-axis CCC machinery where the rotation locus on the rotor is perpendicular to the rotation plane of the column [9,10], (b) adoption of toroidal column on synchronous J-type CCC machinery [7,11], (c) construction of various spiral columns on J-type machinery [2,12], and (d) development of non-synchronous J-type CCC machinery [13,14]. In parallel, use of centrifugal partition chromatography (CPC) for protein separation using ATPS underwent a period of enthusiasm as well [15].
5-HT <inf>2A/C</inf> receptors mediate the antipsychotic-like effects of alstonine
2012, Progress in Neuro-Psychopharmacology and Biological PsychiatryCitation Excerpt :The sample solution was prepared by dissolving 15.0 g of alkaloid fraction of the methylene chloride extract of P. nitida in 100 mL of a phase mixture consisting of equal volumes of each phase. The separation was initiated by completely filling the column with the stationary phase (LC pump) before loading the sample; the mobile phase was pumped into the column at 2 mL/min while the column was rotated at 834 rev/min in the combined head to tail elution mode (Shinomiya et al., 1993). The absorbance of the eluate was continuously monitored at 280 nm and 4 mL fractions were collected.
Scale-up of protein purifications using aqueous two-phase systems: Comparing multilayer toroidal coil chromatography with centrifugal partition chromatography
2011, Journal of Chromatography ACitation Excerpt :Albertsson devoted over 10 pages of his book to this subject and supported the development of multistage unit gravity [1] and centrifugal [2] counter-current distribution systems to enhance their separation. Since then a number of groups internationally have been using ATPS for protein separations using a variety of different counter-current chromatography (CCC) methods to achieve multistage separations: centrifugal partition chromatography (CPC) [3–5], toroidal coil chromatography (TCC) [6–10], non-synchronous CCC [11–15], cross-axis CCC [16–29]; locular CCC [30], spiral disk CCC [31–36] and conventional multilayer CCC [37–39]. These methods often used ATPS but occasionally use single physiological saline solutiuons [40] or other types of phase system [41–44].
The three-dimensional model for helical columns on type-J synchronous counter-current chromatography
2011, Journal of Chromatography ACitation Excerpt :The type-J CCC device has been extensively compared with the other types (e.g. cross-axis, type-I and non-synchronous) over the past 30 years. Interestingly, its importance has been strengthened rather than weakened as a result of these comparisons, with even increasing applications and device sizes [1–6]. For the column geometry, the multi-layer helical column (Fig. 1A) stood out from all the known types of column geometry (e.g. toroidal and spiral columns) early on [3,7–10].
Binary concepts and standardization in counter-current separation technology
2009, Journal of Chromatography A
- 1
Visiting Scientist from College of Pharmacy, Nihon University, 7-7-1, Narashinodai, Funabashi-shi, Chiba 274, Japan.
- 2
Guest scientist from Laboratoire de Chimie Analytique, Ecole Supérieure de Physique et de Chimie Industrielles de Paris, 10 Rue Vauquelin, 75231 Paris Cedex 05, France.