Short communicationHigh-performance liquid chromatographic separation of cis—trans isomers of proline-containing peptides: II. Fractionation in different cyclodextrin systems
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Cited by (25)
High-performance liquid chromatography and nuclear magnetic resonance study of linear tetrapeptides and octapeptides containing N-methylated amino acid residues
2007, Journal of Chromatography ACitation Excerpt :It was found that temperature, flow-rate, pH, and solvent can significantly affect the elution profile of proline containing peptides. It was shown that raising of the column temperature had a dramatic effect on the peak shape, since it changed from a broad or split to uniform sharp profile as a consequence of the accelerated cis/trans isomerism [15–17,20–25,27–29]. On the other hand, low temperature leads to a slower cis/trans interconversion resulting in the peak broadening and, in extreme cases, peak splitting [15,23,28].
Determination of the interconversion energy barrier of enantiomers by separation methods
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2000, Journal of Chromatography AChapter 12 Peptides
1998, Journal of Chromatography Library
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