Determination of platinum binding bases in oligonucleotides. Application of exonuclease digestion method
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Nuclease digestion and mass spectrometric characterization of oligodeoxyribonucleotides containing 1,2-GpG, 1,2-ApG, and 1,3-GpXpG cisplatin intrastrand cross-links
2013, Clinica Chimica ActaCitation Excerpt :Fig. 8 displays the products housing the cisplatin remnant that we identified from LC–MS/MS analysis (Supplementary Fig. S7-S13). As was previously reported with unlabeled cisplatin, we observed m/z values corresponding to cisplatin-chelated (*) 1,2-GpG dinucleoside digestion products harboring one [structure II, d(G*pG*)] [9,11,12,16,17,20,22,31] or two [structure I, d(pG*pG*) or d(G*pG*p)] [13,15,18,19,22,31–35] phosphate groups. Similarly, a cisplatin-containing 1,2-ApG dinucleoside digestion products bearing one [structure IV, d(A*pG*)] or two [structure III, d(pA*pG*) or d(A*pG*p)] [11–13,15,17–20] phosphate groups were observed.
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