Elsevier

Carbohydrate Research

Volume 110, Issue 2, 1 December 1982, Pages 245-259
Carbohydrate Research

Can cyclodextrin glycosyltransferase be useful for the investigation of the fine structure of amylopectins?: Characterisation of highly branched clusters isolated from digests with potato and maize starches

https://doi.org/10.1016/0008-6215(82)84006-8Get rights and content

Abstract

Maize and potato amylopectin (57 and 64%, respectively) were recovered as non-cyclic products from 4-h digests of the starches with cyclodextrin glycosyltransferase {(1→4)-α-d-glucan:[(1→4)-α-d-glucopyranosyl]transferase (cyclising), EC 2.4.1.19} from Klebsiella pneumoniae M 5 al. Besides smaller saccharides, highly branched fragments of different sizes (average d.p. 40–140) were obtained by fractionation. The extents of beta-amylolysis varied between 24 and 37%, indicating that the clusters were not equally susceptible to attack by cyclodextrin glycosyltransferase. The fragments of potato amylopectin still contained larger amounts of material of high molecular weight. Accordingly, part of the longer B-chains of the basic structure were protected from the enzymic attack, presumably because of interchain branches. By debranching with pullulanase, it was evident that the beta-limit dextrins of the fragments of potato amylopectin were composed of longer B-chains (average chainlength 17.8) than those of maize amylopectin (average chain-length 14.1). The A/B-chain ratios, which were calculated from h.p.l.c. data for the debranched beta-limit dextrins, were 1.22 (maize) and 1.06 (potato). Some structural differences between potato and maize amylopectin are discussed.

References (51)

  • E.Y.C. Lee et al.

    Arch. Biochem. Biophys.

    (1968)
  • J.J. Marshall et al.

    Arch. Biochem. Biophys.

    (1974)
  • D.J. Manners et al.

    Carbohydr. Res.

    (1981)
  • Z. Gunja-Smith et al.

    FEBS Lett.

    (1970)
  • H. Akai et al.

    Biochim. Biophys. Acta

    (1971)
  • E.Y.C. Lee et al.

    Arch. Biochem. Biophys.

    (1966)
  • M. Yamaguchi et al.

    J. Ultrastruct. Res.

    (1979)
  • Y. Ikawa et al.

    Carbohydr. Res.

    (1978)
  • M. Abdullah et al.

    Arch. Biochem. Biophys.

    (1970)
  • R.S. Hall et al.

    Carbohydr. Res.

    (1980)
  • K. Umeki et al.

    Carbohydr. Res.

    (1981)
  • D. Borovsky et al.

    Arch. Biochem. Biophys.

    (1979)
  • H. Bender

    Carbohydr. Res.

    (1982)
  • J. Larner et al.

    J. Biol. Chem.

    (1952)
  • H. Bender et al.

    Methods Enzymol.

    (1966)
  • H. Bender

    Anal. Biochem.

    (1981)
  • B.S. Enevoldsen et al.

    FEBS Lett.

    (1977)
  • K. Yokobayashi et al.

    Biochim. Biophys. Acta

    (1970)
  • N. Nelson

    J. Biol. Chem.

    (1944)
  • J.B. Lloyd et al.

    Anal. Biochem.

    (1969)
  • G.K. Adkins et al.

    Carbohydr. Res.

    (1969)
  • H. Bender

    Carbohydr. Res.

    (1980)
  • S. Kobayashi et al.

    Carbohydr. Res.

    (1978)
  • D. French

    Adv. Carbohydr. Chem.

    (1957)
  • G.B. Wells et al.

    Anal. Biochem.

    (1979)
  • Cited by (0)

    1

    Present address: Chinoin Pharmaceutical and Chemical Works Ltd, 1325 Budapest, Hungary.

    View full text