Elsevier

Carbohydrate Research

Volume 178, Issue 1, 15 July 1988, Pages 111-120
Carbohydrate Research

Structures of the eight- to nine-sugar glycolipids of human blood group a erythrocytes

https://doi.org/10.1016/0008-6215(88)80105-8Get rights and content

Abstract

Two glycolipid fractions, isolated in 1975 from blood group A1 erythrocytes and shown on the basis of direct-inlet mass spectrometry to contain eight- and nine-sugar A-type sequences, have been reinvestigated by fast-atom-bombardment mass spectrometry and overlay analysis with selected monoclonal anti-A antibodies. The presence of three separate glycolipids was concluded, consistent with a common paragloboside backbone [β-d-Galp-(1→4)-β-d-GlcpNAc-(1→3)-β-d-Galp-(1→4)-d-Glc] and a typical erythrocyte ceramide component (sphingosine, and 22-, 23-, 24-, and 25-carbon nonhydroxy fatty acids). It is proposed that they carry A determinants based on Type 1 [β-d-Galp-(1→3)-β-d-GlcpNAc], Type 2 [β-d-Galp-(1→4)-β-d-GlcpNAc], and Type 3 [β-d-Galp-(1→3)-α-d-GalpNAc] chains, respectively. The Type 1 (eight sugars) and Type 3 (nine sugars) glycolipids appeared in mixtures of both the native and the acetylated form. The existence of Type 1 glycolipid, which appears to be a genuine erythrocyte glycolipid as concluded from the ceramide composition, had been predicted earlier by other workers.

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