Preparation and assay of chalcone synthase from walnut tree tissue
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Polyphenol metabolism provides a screening tool for beneficial effects of Onobrychis viciifolia (sainfoin)
2012, PhytochemistryCitation Excerpt :Therefore the presence of the main enzymes of the polyphenol pathway as well as modifying enzymes was expected in tissues of O. viciifolia. Preparations from O. viciifolia, which were obtained by standard methods (Claudot and Drouet, 1992; Stich et al., 1992) showed no or only low enzyme activities. An optimised extraction method for polyphenol rich material (Dellus et al., 1997) could be successfully adapted for tissues of O. viciifolia (refer to Section 3.5) and provided preparations showing high activities for all tested enzymes.
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