Elsevier

Phytochemistry

Volume 31, Issue 10, October 1992, Pages 3377-3380
Phytochemistry

Preparation and assay of chalcone synthase from walnut tree tissue

https://doi.org/10.1016/0031-9422(92)83689-VGet rights and content

Abstract

Chalcone synthase activity was obtained from bark tissue of one-year-old shoots taken from a 30-year-old hybrid walnut tree Juglans nigra §x J. regia. High tissue concentrations of phenolics, which are a major problem in woody plant enzyme extraction, were essentially overcome by the use of 200 mM ascorbic acid, 1.5% (w/v) polyethyleneglycol and 50 mM cysteine in the buffers, and a constant flow of nitrogen. Some properties of walnut tree chalcone synthase are discussed in comparison to those of the same enzyme from other plants.

References (24)

  • W. Heller et al.

    Arch. Biochem. Biophys.

    (1980)
  • N. Saleh et al.

    Phytochemistry

    (1978)
  • I.M. Whitehead et al.

    Biochim. Biophys. Acta

    (1983)
  • F. Kreuzaler et al.

    Arch. Biochem. Biophys.

    (1975)
  • G. Hrazdina et al.

    Arch. Biochem. Biophys.

    (1976)
  • K. Koundal et al.

    Phytochemistry

    (1983)
  • L. Britsch et al.

    Phytochemistry

    (1985)
  • W.D. Loomis

    Meth. Enzymol.

    (1969)
  • J. Schröder et al.

    Plant Sci. Letters

    (1979)
  • W. Hinderer et al.

    Phytochemistry

    (1983)
  • R. Spribille et al.

    Phytochemistry

    (1982)
  • W. Hinderer et al.

    Arch. Biochem. Biophys.

    (1985)
  • Cited by (18)

    • Polyphenol metabolism provides a screening tool for beneficial effects of Onobrychis viciifolia (sainfoin)

      2012, Phytochemistry
      Citation Excerpt :

      Therefore the presence of the main enzymes of the polyphenol pathway as well as modifying enzymes was expected in tissues of O. viciifolia. Preparations from O. viciifolia, which were obtained by standard methods (Claudot and Drouet, 1992; Stich et al., 1992) showed no or only low enzyme activities. An optimised extraction method for polyphenol rich material (Dellus et al., 1997) could be successfully adapted for tissues of O. viciifolia (refer to Section 3.5) and provided preparations showing high activities for all tested enzymes.

    View all citing articles on Scopus
    View full text