Biochemical and Biophysical Research Communications
Role of associated and covalently bound lipids in salivary mucin hydrophobicity: Effect of proteolysis and disulfide bridge reduction
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A comparison between the structures of reconstituted salivary pellicles and oral mucin (MUC5B) films
2021, Journal of Colloid and Interface ScienceCitation Excerpt :However, mucins are not the only lubricious component of salivary pellicles [21]. Moreover, mucins have a tendency to interact with other biological molecules [22,23], and it is known that the lubrication performance of mucin films can be improved, becoming closer to that of salivary pellicles, if mixed with other salivary fractions [17]. Thus, while a number of works indicate that an outer layer of anchored oral mucins mixed with other salivary components mediates the highly efficient boundary lubrication exhibited by reconstituted salivary pellicles, the nature of these additional pellicle components and the mechanisms by which they influence the structure and lubrication properties of mucins remains unknown.
Sulfate-based lipids: Analysis of healthy human fluids and cell extracts
2019, Chemistry and Physics of LipidsCitation Excerpt :In fact, targeted MS-based approaches have been widely used for the SL detection but also due to their ability for structural characterization by tandem mass spectrometry (MS/MS) experiments. Despite the many studies conducted on the identification of SL in plasma (Lee et al., 2016; Mirzaian et al., 2015), saliva (Slomiany et al., 1988, 1983, 1978), urine (Yao et al., 2016), tear fluid (Lam et al., 2014), gastric fluid (Iwamori et al., 2000), seminal plasma (Sion et al., 2001), lipoproteins (Reis et al., 2013; Serna et al., 2015), red blood cells (Muskiet et al., 1983; Przybylska et al., 1995), blood platelets (Blache et al., 1995), and tissues brain (Moyano et al., 2013), kidney (Tadano-Aritomi et al., 2001), eye lenses (Seng et al., 2014), sperm cells (Fujimoto et al., 2000; Kongmanas et al., 2010; Zhang et al., 2005), hair (Cho et al., 2010; Drosche et al., 1994; Serizawa et al., 1990) and nails (Serizawa et al., 1990) and quantification by multi-reaction monitoring (MRM) (Fong et al., 2013; Kongmanas et al., 2010; Moyano et al., 2013; Sánchez-Guijo et al., 2015; Yao et al., 2016), these were mostly focused on specific steroid sulfates in individual fluids and cells (Esquivel et al., 2018; Fong et al., 2013; Lam et al., 2014; Przybylska et al., 1995; Sánchez-Guijo et al., 2015; Sion et al., 2001; Wang et al., 2018) leaving a whole panel of high Mw SL unaccounted for. In this study, the optimisation of targeted PIS 97 detection conditions to include low and high Mw SL (Figs. 1 and 2) revealed the presence of sulfate steroids, sulfoglycolipids, sulfated hypurate lipids, N-acyl taurine lipids and other unidentified sulfate lipid-based compounds identified in the same mass spectra (Fig. 3).
Gel-forming mucin interactome drives mucus viscoelasticity
2018, Advances in Colloid and Interface ScienceCitation Excerpt :By contrast, other reports have noted relatively abundant lipids in mucus gels (1–2% of the total weight). Several classes of lipids have been described, including neutral lipids, the major class with more than half of the lipid fraction, and to a lesser extent glycolipids and phospholipids [114–116]. Their role is presumably to create a hydrophobic barrier at the mucus layer surface.
What interactions drive the salivary mucosal pellicle formation?
2014, Colloids and Surfaces B: BiointerfacesCitation Excerpt :This heavily glycosylated protein is thought to be an essential part of the enamel and mucosal pellicle [7] and with its gel forming properties is thought to be essential for lubrication in the oral cavity [15]. MUC5B has both hydrophilic domains and hydrophobic domain patches [12,30], however its hydrophobic domains are within the non-glycosylated region and it is possibly covalently bound lipids from saliva that contribute to its hydrophobic nature [34]. Its low level of binding may also be due to the lack of membrane bound MUC1 on the particle surfaces [35,36] which could be essential in the binding of MUC5B to the mucosa in the oral cavity and development of the pellicle.
Salivary phospholipid secretion in response to β-adrenergic stimulation is mediated by Src kinase-dependent epidermal growth factor receptor transactivation
2004, Biochemical and Biophysical Research CommunicationsMechanisms of oesophageal mucosal defence
2000, Bailliere's Best Practice and Research in Clinical Gastroenterology