Perturbed γ-γ angular correlation studies of 111in bound to double and single stranded DNA

doi:10.1016/0020-708X(84)90026-7

The International Journal of Applied Radiation and Isotopes

Volume 35, Issue 9, September 1984, Pages 889-893

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Abstract

Using ¹¹¹In as a probe, the dynamics of double and single stranded DNA have been studied by performing time-differential γ-γ angular correlation measurements on the 173–247 keV transitions in ¹¹¹Cd. Binding of indium to DNA was investigated by analysis of sedimentation profiles, thermal transition spectrophotometry and electrochromatography. Rotational correlation times τ_c⩾400 ns and τ_c = 27 ± 7 ns were obtained respectively for double stranded (native) and single stranded (denatured) DNA. The corresponding quadrupole interaction frequencies were ω₀ = 711 ± 15 Mrad/s and ω₀ = 151 ± 13 Mrad/s.

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Perturbed γ-γ angular correlation studies of 111in bound to double and single stranded DNA

Abstract

Nucl. Instrum. Methods

Chem. Phys. Lett.

J. Chem. Phys.

Methods Enzymol.

J. Mol. Biol.

J. Chem. Phys.

J. Chem. Phys.

Phys. Rev.

J. Chem. Phys.

Perturbed γ-γ angular correlation studies of ¹¹¹in bound to double and single stranded DNA