Regular ArticleThe Lactate/Pyruvate Shuttle in Spermatozoa: Operation in Vitro
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Pyruvate modulation of redox potential controls mouse sperm motility
2024, Developmental CellThe effects of metabolic substrates glucose, pyruvate, and lactate added to a skim milk-based semen extender for cooled storage of stallion sperm
2021, TheriogenologyCitation Excerpt :This concentration of lactate is similar to that commonly found in various sperm culture media (i.e. Biggers Whitten’s Whittingham – BWW, or Tyrode’s Lactate Pyruvate – TALP; [11]). Evidence suggests that pyruvate or lactate might be easily incorporated into the mitochondrial matrix of equine or rodent sperm [11,12], likely via lactate or pyruvate shuttles located at the mitochondrial membrane. This would subsequently result in the production Acetyl-CoA and the proton carriers NADH and FADH2 required for normal occurrence of OXPHOS at the mitochondrial electron transport chain [10,11,31,37].
The sperm mitochondrion: Organelle of many functions
2018, Animal Reproduction ScienceThe Science and Translation of Lactate Shuttle Theory
2018, Cell MetabolismCitation Excerpt :Thus as in mitochondrial preparations from other mammalian tissues (Brooks et al., 1999a; Butz et al., 2004), both MCT and LDH are required for the oxidation of lactate by sperm mitochondrial preparations. Those results on boar sperm mitochondria were supported by results on sperm mitochondria from rats and rabbits (Gallina et al., 1994). To be complete, it should be noted that although available data support the presence of a lactate shuttle in mitochondria from boars, rats, rabbits, and cattle, the lactate shuttle is not likely present in mouse spermatozoa (Gallina et al., 1994), and both lactate and malate-aspartate shuttles are present in spermatozoa of many species (Calvin and Tubbs, 1978), which is common in cells of many tissues from diverse mammalian species.
Differences in ATP generation via glycolysis and oxidative phosphorylation and relationships with sperm motility in mouse species
2015, Journal of Biological ChemistryCitation Excerpt :Furthermore, the GLUT8 glucose transport facilitator has been found in the mouse sperm midpiece where it is required for the maintenance of mitochondrial membrane potential (50). Regardless of whether lactate (51–53) or pyruvate (18, 34, 54) is the primary respiratory substrate entering mouse sperm mitochondria, the composition of the medium used in our experiments made active glycolysis a prerequisite for OXPHOS. Although the physiological (in vivo) relevance of the uptake by mitochondria of glycolytic products excreted by the principal piece is uncertain, the different regions of the mouse female reproductive tract (vagina, uterus, and oviduct) present relatively high lactate/glucose ratios around the time of ovulation (2, 7) that would enable sperm to import sufficient exogenous lactate to fuel OXPHOS (52, 53, 55, 56).